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Epidemiological analysis of Leishmaniasis prevalence in Pakistan during 2016-2023.
Braz J Biol
January 2025
Near East University, Operational Research Center in Healthcare, Mersin, Turkey.
Leishmaniasis, caused by the Leishmania parasite, remains a persistent public health challenge in Pakistan. Despite control efforts, the disease prevalence continues to rise, particularly among pediatric populations. Understanding prevalence patterns and transmission dynamics is critical for effective control strategies.
View Article and Find Full Text PDFInsights and Opportunities from Multimarker Evaluation of Heart Failure: Lessons from BIOSTAT-HF.
Curr Heart Fail Rep
January 2025
Division of Cardiovascular Medicine, Department of Medicine, University of California, 9394 Medical Center Drive, La Jolla, San Diego, CA, USA.
Purpose Of Review: Heart failure is a complex and heterogenous disease state that affects millions worldwide. Over recent decades, advancements in medical therapy and device implementation have significantly transformed the landscape of heart failure outcomes, while improvements in imaging modalities and greater accessibility to genome sequencing have led to increasing recognition of distinct heart failure endotypes. There is rising evidence to suggest all patients do not benefit equally from intensification of guideline directed medical therapy (GDMT).
View Article and Find Full Text PDFThe relationship between stress, social support, and quality of life among spouses of patients at the end-of-life stage in Thailand: A correlational study.
Belitung Nurs J
January 2025
Ramathibodi School of Nursing, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Thailand.
Background: Non-communicable diseases have become a leading global health challenge, with mortality rates steadily rising. Insufficient patient care can worsen chronic illness, often placing significant caregiving responsibilities on the patient's spouse. In some cases, spouses may need to leave their jobs to provide full-time care, especially during the end-of-life stage.
View Article and Find Full Text PDFThe rising prevalence of obesity and diabetes underscores the need for innovative approaches to promote healthier lifestyles and improve clinical outcomes. Emerging evidence suggests that integrating mobile health (mHealth) technologies, such as smartphone applications and wearable devices, may provide a promising solution. mHealth interventions have the potential to enhance the delivery and accessibility of nutritional therapy and lifestyle modification programs for people with obesity and diabetes.
View Article and Find Full Text PDFPeptide nucleic acid (PNA) clamps reduce amplification of host chloroplast and mitochondria rRNA gene sequences and increase detected diversity in 16S rRNA gene profiling analysis of oak-associated microbiota.
Environ Microbiome
January 2025
School of Natural Sciences, Bangor University, Bangor, UK.
Background: Acquiring representative bacterial 16S rRNA gene community profiles in plant microbiome studies can be challenging due to the excessive co-amplification of host chloroplast and mitochondrial rRNA gene sequences that reduce counts of plant-associated bacterial sequences. Peptide Nucleic Acid (PNA) clamps prevent this by blocking PCR primer binding or binding within the amplified region of non-target DNA to stop the function of DNA polymerase. Here, we applied a universal chloroplast (p)PNA clamp and a newly designed mitochondria (m)PNA clamp to minimise host chloroplast and mitochondria amplification in 16S rRNA gene amplicon profiles of leaf, bark and root tissue of two oak species (Quercus robur and Q.
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