Digital holographic microscopy for real-time investigation of 3D microstructural dynamics of starch-kefiran-based nanocomposite.

Investigating real-time phenomena in bio-polymers has received much attention because of their increasing demands in polymer substitution. The 3D morphometry of polymer surfaces may be very impactful in such studies. Here, digital holographic microscopy (DHM) is applied for quantitative measurement of the rare morphological changes of UV-A and UV-C exposed nanocomposites during their incubation with excess water. By reconstructing the recorded successive digital holograms, the time evolution of the swelled regions of the samples is derived. Our results clearly show that the higher water swelling of UV-A irradiated starch/kefiran/ZnO may be attributed to its higher hydrophilicity.