B cells express a unique antibody protein which comprises two pairs of immunoglobulin (Ig) heavy (H) and light (L) chains. In addition to an invariable constant (C) region, IgH and IgL chains encompass a variable (V) region mediating antigen binding. This unique region stems from Ig V(D)J gene recombination, which generates diversity by assembling these gene segments into VDJ and VJ genes. To ensure that one B cell only expresses one antibody, VDJ rearrangement occurs only in one IgH locus (allelic exclusion), whereas VJ rearrangement only in either the κ or λ locus (isotype exclusion). However, teleosts express multiple IgLs encoded by distinct C genes. Using single-cell transcriptomics, we have demonstrated the transcription of distinct rearranged VJC genes in single rainbow trout B cells. Our results highlight the laxity of isotype exclusion in teleosts and strongly suggest that fish B cells can produce antibodies of different specificities.
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| http://dx.doi.org/10.1016/j.isci.2021.102615 | DOI Listing |
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