AI Article Synopsis
- CD34 hematopoietic stem and progenitor cells were obtained from a 49-year-old male and successfully converted into human induced pluripotent stem cells (hiPSCs) using integration-free methods.
- The resulting hiPSC line demonstrated typical stem cell characteristics, including the expression of key pluripotency markers and the absence of reprogramming vectors.
- The cell line was confirmed to maintain genomic integrity and showed capability to differentiate into all three germ layers, making it a valuable control for studying diseases.
Article Abstract
Peripheral-blood derived CD34 hematopoietic stem and progenitor cells were isolated from a 49-year old male donor and were successfully reprogrammed into human induced pluripotent stem cells (hiPSCs) using integration-free episomal vectors. The hiPSC line exhibited a typical stem cell-like morphology and endogenously expressed several pluripotency markers by concomitant loss of exogenous reprogramming vectors. Genomic integrity was confirmed by microarray-based comparative genomic hybridization (array CGH). Further analysis affirmed the ability of this hiPSC line to differentiate into all three germ layers. Thus, the reported cell line may serve as a healthy control for disease modeling.
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| http://dx.doi.org/10.1016/j.scr.2021.102427 | DOI Listing |
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