AI Article Synopsis
- The use of ion-air reagents in reversed-phase chromatography helps separate purine and pyrimidine derivatives effectively.
- Simultaneous analysis of nucleotides, nucleosides, and their bases was performed using an acetonitrile gradient and tetrabutyl ammonium phosphate as a counterion.
- Optimal conditions for the isocratic separation of adenine nucleotides and metabolic products were established, along with modifications to separate specific purines and pyrimidines.
Article Abstract
Utilization of ion-air reagents in a reversed-phase chromatographic system allows solving a number of problems related to the separation of purine and pyrimidine derivatives. Simultaneous analysis of nucleotides, nucleosides and their bases was carried out by acetonitrile gradient elution using tetrabutyl ammonium phosphate as a counterion in the mobile phase. Besides, optimal conditions were selected for isocratic separation of adenine nucleotides and their metabolites. Furthermore, isocratic separation of certain purines and pyrimidines was achieved by modifying the stationary C18-phase with pentane- and heptane sulphonic acids.
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