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http://dx.doi.org/10.1007/s40820-021-00608-4 | DOI Listing |
ACS Appl Mater Interfaces
January 2025
Faculty of Life Sciences, Department of Pharmaceutical Sciences, Laboratory of Macromolecular Cancer Therapeutics (MMCT), University of Vienna, Josef-Holaubek-Platz 2, 1090 Vienna, Austria.
Splice-switching oligonucleotides (SSOs) can restore protein functionality in pathologies and are promising tools for manipulating the RNA-splicing machinery. Delivery vectors can considerably improve SSO functionality in vivo and allow dose reduction, thereby addressing the challenges of RNA-targeted therapeutics. Here, we report a biocompatible SSO nanocarrier, based on redox-responsive disulfide cross-linked low-molecular-weight linear polyethylenimine (cLPEI), for overcoming multiple biological barriers from subcellular compartments to en-route serum stability and finally in vivo delivery challenges.
View Article and Find Full Text PDFBio Protoc
January 2025
University of Bordeaux, CNRS, IBGC UMR 5095, Bordeaux, France.
Stable-isotope resolved metabolomics (SIRM) is a powerful approach for characterizing metabolic states in cells and organisms. By incorporating isotopes, such as C, into substrates, researchers can trace reaction rates across specific metabolic pathways. Integrating metabolomics data with gene expression profiles further enriches the analysis, as we demonstrated in our prior study on glioblastoma metabolic symbiosis.
View Article and Find Full Text PDFNat Biomed Eng
January 2025
Department of Neurosurgery, University of Texas Medical Branch, Galveston, TX, USA.
J Neural Eng
January 2025
Department of Information Engineering, Electronics and Telecommunications, University of Rome La Sapienza, Piazzale Aldo Moro 5, Rome, 00185, ITALY.
Deep learning tools applied to high-resolution neurophysiological data have significantly progressed, offering enhanced decoding, real-time processing, and readability for practical applications. However, the design of artificial neural networks to analyze neural activity in vivo remains a challenge, requiring a delicate balance between efficiency in low-data regimes and the interpretability of the results. Approach: To address this challenge, we introduce a novel specialized transformer architecture to analyze single-neuron spiking activity.
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