The antimesometrial part of rat's decidua of the 9th day of gestation was divided into three zones. Cells of either zone display their own morphological and cytochemical properties. Different rates of 3H-uridine incorporation were observed in the cytoplasm and the nucleus in cells of either zone during 5, 30, 60 and 240 minutes after precursor injection. The largest member of silver grain accumulation was observed in the karyoplasm and nucleolus of cells of the transitional zone. The nucleus of basal zone cells had the smallest intensity of 3H-uridine incorporation. The nuclei of the epithelial zone cells are characterized by a lower intensity of 3H-uridine incorporation than those of the transition zone. The intensity to cytoplasmic accumulation of silver grains raised from cells of the basal zone up to cells of the epithelial zone. The largest quantity of cytoplasmic radioactivity was observed 240 minutes after 3H-uridine injection.
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Curr Radiopharm
January 2012
Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Matsumoto, Japan.
For the purpose of studying the aging changes of macromolecular synthesis in the pancreatic acinar cells of experimental animals, we studied 10 groups of aging mice during development and aging from fetal day 19 to postnatal month 24. They were injected with 3H-uridine, a precursor for RNA synthesis, sacrificed and the pancreatic tissues were taken out, fixed and processed for light and electron microscopic radioautography. On many radioautograms the localization of silver grains demonstrating RNA synthesis in pancreatic acinar cells in respective aging groups were analyzed qualitatively.
View Article and Find Full Text PDFJ Exp Clin Cancer Res
December 2010
Department of Anticancer Drug Development, Chittaranjan National Cancer Institute, Kolkata 700026, India.
Background: Anticancer activities of several substituted naphthalimides (1H-benz[de]isoquinoline-1,3-diones) are well documented. Some of them have undergone Phase I-II clinical trials. Presently a series of ten N-(hydroxyalkyl) naphthalimides (compounds 1a-j) were evaluated as antitumor agents.
View Article and Find Full Text PDFInt J Oncol
March 2010
Anti-Cancer Drug Laboratory, Division of Biology, Ackert Hall, Kansas State University, Manhattan, KS 66506-4901, USA.
Substituted quinolines (PQ code number), which reduce colony formation and increase gap junctional intercellular communication, were tested for their ability to interact with various molecular targets in murine and human tumor cell lines in vitro. Various markers of tumor cell metabolism, DNA fragmentation, mitotic disruption, apoptosis induction and growth factor receptor signaling pathways were assayed in vitro to evaluate drug cytotoxicity. Based on its ability to inhibit the metabolic activity of suspension cultures of leukemic L1210 cells at days 2 and 4 in vitro, PQ1 succinic acid salt is the most effective antiproliferative agent among the synthetic quinoline analogs tested.
View Article and Find Full Text PDFBiochim Biophys Acta
October 2009
Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas, Chacabuco 461. San Miguel de Tucumán T4000ILI, Argentina.
Background: Microcin J25 targets the RNA polymerase as well as bacterial membranes. Because there is scarce information on the relationship between the uptake and the activity, a fluorescent microcin J25-derivative was used to further characterize its mechanism of action.
Methods: MccJ25 I13K was labeled with FITC and its uptake by sensitive cells was assessed by fluorescence measurements from supernatants of MccJ25-Escherichia coli suspensions.
Using 3H-uridine dynamics of RNA synthesis in oocytes of tilapia which grow at different temperatures was studied. Intact fishes were cultivated at the temperature 25-26 degrees C, experimental ones -- 19-21 degrees C. Before 3H-uridine injection, experimental fishes were cultivated at low temperature for 5 days, then both intact and experimental fishes were injected by 3H-uridine and experiment was continued for 5 days.
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