Similar Publications

Conjugation of radiofluorinated prosthetic groups to primary amines of peptides in an aqueous medium is still considerably challenging. Herein, we report a one-pot cascade synthesis of 1-[F]fluoro-4-isothiocyanatobenzene ([F]), an isothiocyanate-functionalized prosthetic group for radiolabeling of various peptides in aqueous medium. The developed compound [F] was synthesized in >99% radiochemical purity with 22.

View Article and Find Full Text PDF

Golden Gate Assembly of Transcriptional Unit Libraries into a Rearrangeable Gene Cluster.

Methods Mol Biol

October 2024

European Molecular Biology Laboratory (EMBL), Genome Biology Unit, Heidelberg, Germany.

Both regulatory sequences and genome organization contribute to the production of diverse transcript isoforms, which can influence how genes, or sets of genes, are expressed. An efficient, modular approach is needed to generate the combinatorial complexity required to empirically test many combinations of different regulatory sequences and different gene orders. Golden Gate assembly provides such a tool for seamless one-pot cleavage and ligation, by using type IIS restriction enzymes, which cleave outside of their recognition site.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!