Characterization of the Complete Mitochondrial Genome of Basidiomycete Yeast : Intron Evolution, Gene Rearrangement, and Its Phylogeny.

In this study, the mitogenome of was sequenced by next-generation sequencing (NGS) and successfully assembled. The mitogenome comprised circular DNA molecules with a total size of 26,444 bp. We found that the mitogenome of partially deleted the tRNA gene transferring cysteine. Comparative mitogenomic analyses showed that intronic regions were the main factors contributing to the size variations of mitogenomes in Tremellales. Introns of the gene in Tremellales species were found to have undergone intron loss/gain events, and introns of the gene may have different origins. Gene arrangement analysis revealed that contained a unique gene order different from other Tremellales species. Phylogenetic analysis based on a combined mitochondrial gene set resulted in identical and well-supported topologies, wherein was closely related to . This study represents the first report of mitogenome for the genus, which will allow further study of the population genetics, taxonomy, and evolutionary biology of this important phylloplane yeast and other related species.