Interactions of polyphenols from traditional apple varieties 'Bobovac', 'Ljepocvjetka' and 'Crvenka' with β-Glucan during in vitro simulated digestion.

Interactions between β-glucan and polyphenols might have an effect on polyphenol digestion and bioaccessibility. The influence of β-glucan on in vitro gastrointestinal digestion of polyphenols of traditional apple varieties was studied. Polyphenols were chemically and enzymatically extracted, and identified and quantified with high-performance liquid chromatography. Simulated digestion of peel and flesh of apples was conducted. Polyphenols released in digestion in lower amounts than occur naturally in apples. Their content increased from the oral to the gastric, then decreased in the intestinal phase (up to 21% (peel) and 16% (flesh) were recovered) where anthocyanins and flavan-3-ols were not found. β-glucan decreased (oral and intestinal digestion of peel) or increased (gastric digestion of peel; oral, gastric, intestinal digestion of flesh) the recovered polyphenols. Interactions between β-glucan, polyphenols and enzymes might have influenced these effects. β-glucan is suggested to increase the polyphenol content reaching lower parts of the digestive tract.