This study was conducted to elucidate the effects of introducing conjugated linoleic acid (CLA) on meiotic spindle organization of heat stressed (HS) matured oocytes and the resulting blastocysts DNA methylation as well as the expression of the genes involved in DNA methylation (DNMT3a, DNMT3b and DNMT1). Immature bovine oocytes were cultured at 41 °C for the first 12 h and 38.5 °C for the second 12 h of maturation time in the presence of 0 and 50 μM of CLA (HS and HS + CLA groups, respectively). A group of oocytes cultured in medium with no CLA supplementation at normal temperature (38.5 °C for 24 h) was considered as negative control (C). Percentage of normal spindle, and cleavage and blastocyst rates were significantly decreased in the HS group compared to the C group (P < 0.05). The global DNA methylation and expression level of DNMT3a gene were increased in HS group compared to the C groups (P < 0.05), while the expression level of DNMT3b was decreased. The CLA supplementation improved the percentage of normal microtubules shape in MII oocytes as well as the developmental competence in the HS + CLA group compared to the HS group (P < 0.05). However, global DNA methylation and expression level of DNMT3a/b were not ameliorated by CLA supplementation (P > 0.05). Based on the obtained results, CLA proved to be capable of improving the oocyte developmental competence as well as decreased the aberrant spindle organization of heat-stressed oocytes and it would not cause epigenetic alteration in the obtained blastocysts.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.theriogenology.2021.05.025 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Isolation of AP1, a rumen bacterium that forms 10,12-conjugated linoleic acid.
JDS Commun
January 2025
Department of Animal Science, University of California, Davis, Davis, CA 95168.
Some microbes in the rumen form 10,12 (10,12)-conjugated linoleic acid (CLA), a fatty acid that depresses synthesis of milk fat in dairy cattle and other lactating animals. Despite their importance to milk fat depression, the microbes responsible have been difficult to identify, and no laboratory strain is currently available for study. Here we describe the isolation of AP1, a bacterium that forms 10,12-CLA at fast rates.
View Article and Find Full Text PDFProtected palmitic acid to mitigate milk fat depression in grazing Holstein cows in the Mexican Highlands.
Arch Anim Nutr
January 2025
Posgrado en Producción Animal. Departamento de Zootecnia, Universidad Autónoma Chapingo, Texcoco, México.
The and isomers of conjugated linoleic acid (CLA) are associated with anticancer and lipolytic effects in tissues, respectively, but in lactating cows, the latter isomer reduces the milk fat concentration, a detrimental aspect for the dairy industry, as it reduces the yield of milk derivatives. Therefore, the objective of this study was to evaluate the effect of providing protected palmitic acid (PA) to grazing lactating Holstein cows supplemented with soybean oil as a source of conjugated linoleic acid, on milk production, fat concentration and mitigation of milk fat depression. Nine multiparous Holstein cows were used, distributed in three groups of three cows each, with initial means of days in milk, live weight, milk production, and number of calvings: 124 ± 16 days, 494 ± 53 kg, 20.
View Article and Find Full Text PDFThe PI4K2A gene positively regulates lipid synthesis in bovine mammary epithelial cells and attenuates the inhibitory effect of t10,c12-CLA on lipid synthesis.
Sci Rep
January 2025
College of Animal Science and Technology, Ningxia Key Laboratory of Ruminant Molecular and Cellular Breeding, Ningxia University, Yinchuan, 750021, China.
Currently, the identification of valuable candidate genes affecting milk fat synthesis in dairy cows is still limited, and the specific regulatory mechanism is still unknown. In this study, we used primary bovine mammary epithelial cells(BMECs)as a model and utilized overexpression and knockdown techniques for the PI4K2A gene to investigate the specific mechanisms by which it regulates lipid metabolism in BMECs. We studied whether PI4K2A regulates the inhibition of trans-10, cis-12 conjugated linoleic acid (t10,c12-CLA) on lipid synthesis in BMECs.
View Article and Find Full Text PDFImpact of dysregulated microbiota-derived C18 polyunsaturated fatty acid metabolites on arthritis severity in mice with collagen-induced arthritis.
Front Immunol
January 2025
Department of Rheumatology and Clinical Immunology, Kobe University Graduate School of Medicine, Kobe, Japan.
Objective: We aimed to evaluate microbiome and microbiota-derived C18 dietary polyunsaturated fatty acids (PUFAs), such as conjugated linoleic acid (CLA), and to investigate their differences that correlate with arthritis severity in collagen-induced arthritis (CIA) mice.
Methods: On day 84 after induction, during the chronic phase of arthritis, cecal samples were analyzed using 16S rRNA sequencing, and plasma and cecal digesta were evaluated using liquid chromatography-tandem mass spectrometry. Differences in microbial composition between 10 control (Ctrl) and 29 CIA mice or between the mild and severe subgroups based on arthritis scores were identified.
This study aimed to investigate the impact of dietary soybean oil and probiotics on goat meat quality, total conjugated linoleic acids (TCLA) concentration, and nutritional quality indicators of goats. Thirty-six male crossbred goats (Anglo-Nubian♂× Thai native♀), weighing 18.3 ± 2.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!