AI Article Synopsis
- The diversity of diseases like cancer necessitates the use of high-throughput screening methods to gather extensive data on tumor characteristics, which aids in predictions and treatment development.
- Microarray technology plays a crucial role in quickly analyzing various biological samples, making it a key tool in biomedical research.
- The text describes protein microarrays based on antibody-antigen interactions and includes a validation method using NAPPA technology to analyze protein functions from screening results.
Article Abstract
The heterogeneity of diseases such as cancer makes it necessary to use high-throughput screening techniques to obtain the maximum number of parameters and characteristics of tumors. These obtained biomarkers can be used for the prediction, prognosis, and treatment or search for new therapeutic targets. In this sense, microarray technology allows exhaustive analysis in a short time and from a great variety of biological samples, becoming a fundamental tool in biomedical research projects. Here, operational process of protein microarrays based on the antibody-antigen interaction is described, emphasizing their application in intracellular signaling pathways in tumoral pathologies. In addition, a final validation using nucleic acid programmable protein array (NAPPA) technology in a simple ELISA assay was included to decipher functional characterization of featured proteins from microarray screening.
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| http://dx.doi.org/10.1007/978-1-0716-1562-1_15 | DOI Listing |
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