sp. nov., a novel actinobacterium isolated from the root of pumpkin ().

A novel acidophilic actinobacterium, designated strain NEAU-YB345, was isolated from a pumpkin root collected from Mudanjiang, Heilongjiang Province, northeast PR China. Based on 16S rRNA gene sequence similarity and chemotaxonomic and morphological properties, the isolate was assigned to the genus , with the high 16S rRNA gene sequence similarities to JCM 16224 (99.2 %), JCM 16223 (99.1 %) and JCM 12277 (98.7 %). Its cell wall contained ll-diaminopimelic acid as the major diamino acid. Rhamnose, ribose, glucose and galactose were the detected sugars from the whole-cell hydrolysates. The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified phospholipid. The menaquinones were MK-9(H) and MK-9(H). Major fatty acids were C, iso-C, iso-C and anteiso-C. Phylogenetic analysis using 16S rRNA gene and whole-genome sequences placed the strain in distinct clades but within the genus . The DNA G+C content was 71.2 mol%. Based on DNA-DNA relatedness and physiological and biochemical data, the isolate could be distinguished from its closest relatives. Therefore, strain NEAU-YB345 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-YB345 (=CCTCC AA 2020030=JCM 33976).