AI Article Synopsis
- SARS-CoV-2 therapeutic antibody discovery has achieved success but is often inefficient, needing many candidates to find just a few viable options.
- The study demonstrates that adding antibody-ligand blocking to the LIBRA-seq platform enhances the efficient identification of powerful neutralizing antibodies against SARS-CoV-2.
- LIBRA-seq with ligand blocking is a versatile method for discovering functional antibodies that target the interruption of antigen-ligand interactions.
Article Abstract
SARS-CoV-2 therapeutic antibody discovery efforts have met with notable success but have been associated with a generally inefficient process, requiring the production and characterization of exceptionally large numbers of candidates for the identification of a small set of leads. Here, we show that incorporating antibody-ligand blocking as part of LIBRA-seq, the high-throughput sequencing platform for antibody discovery, results in efficient identification of ultra-potent neutralizing antibodies against SARS-CoV-2. LIBRA-seq with ligand blocking is a general platform for functional antibody discovery targeting the disruption of antigen-ligand interactions.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183015 | PMC |
| http://dx.doi.org/10.1101/2021.06.02.446813 | DOI Listing |
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