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An atlas of seven zebrafish hox cluster mutants provides insights into sub/neofunctionalization of vertebrate Hox clusters. | LitMetric

AI Article Synopsis

  • Vertebrate Hox clusters, made up of multiple Hox genes, play a critical role in controlling body shape and developmental timing across various axes in the body.
  • This study used the CRISPR-Cas9 technique to create loss-of-function alleles for all seven Hox clusters in zebrafish, allowing for a detailed analysis of their roles.
  • Results showed that while there are unique functional contributions from the Hox clusters in zebrafish, there are also important shared principles observed, such as vertebral changes along the body axis, highlighting the evolutionary variations and implications of Hox genes.

Article Abstract

Vertebrate Hox clusters are comprised of multiple Hox genes that control morphology and developmental timing along multiple body axes. Although results of genetic analyses using Hox-knockout mice have been accumulating, genetic studies in other vertebrates have not been sufficient for functional comparisons of vertebrate Hox genes. In this study, we isolated all of the seven hox cluster loss-of-function alleles in zebrafish using the CRISPR-Cas9 system. Comprehensive analysis of the embryonic phenotype and X-ray micro-computed tomography scan analysis of adult fish revealed several species-specific functional contributions of homologous Hox clusters along the appendicular axis, whereas important shared general principles were also confirmed, as exemplified by serial anterior vertebral transformations along the main body axis, observed in fish for the first time. Our results provide insights into discrete sub/neofunctionalization of vertebrate Hox clusters after quadruplication of the ancient Hox cluster. This set of seven complete hox cluster loss-of-function alleles provide a formidable resource for future developmental genetic analysis of the Hox patterning system in zebrafish.

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Source
http://dx.doi.org/10.1242/dev.198325DOI Listing

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