Analytically pure proteins are indispensable for diverse applications, including therapeutics. Here, we report a methodology where a single amino acid, glycine, enables metal-free protein purification. This robust platform is enabled by a Gly-tag resin for site-specific capture, enrichment, and release through chemically triggered C-C bond dissociation by resonance-assisted electron density polarization.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163197 | PMC |
| http://dx.doi.org/10.1039/d0sc01153c | DOI Listing |
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