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Biogenesis of Outer Membrane Vesicles Concentrates the Unsaturated Fatty Acid of Phosphatidylinositol in . | LitMetric

AI Article Synopsis

  • Bacterial outer membrane vesicles (OMVs) are tiny spherical structures released by bacteria that help in forming oral biofilms, aggregating cells, and enabling communication between them.
  • This study focused on the OMVs produced by a specific bacterium associated with oral biofilms, discovering that their formation is influenced by unsaturated fatty acids and that they are produced through a process called 'blebbing,' not random cell lysis.
  • The research also identified the size and lipid composition of these OMVs and suggested that their protein content remains relatively consistent across different growth phases, indicating their potential role in oral and systemic diseases.

Article Abstract

Bacterial outer membrane vesicles (OMVs) are spherical lipid bilayer nanostructures released by bacteria that facilitate oral biofilm formation cellular aggregation and intercellular communication. Recent studies have revealed that is one of the dominant members of oral biofilms; however, their potential for OMV production has yet to be investigated. This study demonstrated the biogenesis of OMVs in associated with the concentration of unsaturated fatty acids of phosphatidylinositol (PI) and characterized the size and protein profile of OMVs produced at growth phases. Transmission electron microscopy showed isolated spherical structures from cells stained with heavy metals, indicating the production of OMVs with a size ranging from 25 to 100 nm. Lipidome analysis revealed the presence of phosphatidic acid, phosphatidylethanolamine, phosphatidylcholine, and PI as the main lipids. Some unsaturated fatty acids of PI were present specifically in OMV and little in the outer membrane, suggesting that OMVs are generated from a specific region of the membrane through blebbing rather than a random process such as cell lysis. Furthermore, the lack of similar PI accumulation in the OMV of suggests that has a different biogenesis mechanism. The blebbing mechanism was further supported by higher OMV production occurring at the exponential phase in comparison to the stationary phase, where cell lysis is more likely to occur. Further, comparative protein profile of OMVs isolated under different growth phases may indicate that the OMV cargo does not largely vary with growth phases. The present study provides a basis for further understanding the roles of OMVs in oral biofilms as well as systemic diseases that involves.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176214PMC
http://dx.doi.org/10.3389/fmicb.2021.682685DOI Listing

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