1. Pure swards of perennial ryegrass (Lolium perenne cv. Melle) as a primary growth (May), trimmed primary growth (early June) and regrowth (late June), and white clover (Trifolium repens cv. Blanca) as a mature primary growth (July) and vegetative regrowth (August), were grazed by twelve Friesian steers (mean body-weights throughout experiment 152-231 kg) at daily allocation rates of forage which provided dry matter (DM) intakes ranging from 28 to 36 g/kg body-weight). 2. Total nitrogen contents of the three ryegrasses declined with season (37-20 g/kg DM), but in vitro organic matter (OM) digestibilities were relatively constant (0.76-0.79). The clovers had higher N (average 36.6 g/kg DM) and lower fibre and water-soluble carbohydrate contents than the grasses, and in vitro OM digestibilities of 0.70 and 0.76 respectively. 3. Duodenal digesta samples were obtained, using a portable sampling apparatus from the animals grazing the pasture, and estimates of the flow of nutrients into the small intestine were derived using two indigestible markers which were continuously infused into the rumen using a portable infusion apparatus. Forage intakes were calculated from estimates of faecal output of indigestible OM and the predicted in vivo OM digestibilities of the forages consumed. Coefficients of variation for OM flow to the small intestine and OM intake were 11.8 and 10.9% respectively. 4. The apparent digestion of OM in the rumen ranged from 722 to 741 g/kg digestible OM intake and from 681 to 711 g/kg digestible OM intake for the grass and clover diets respectively. Substantial losses of ingested N before the small intestine were measured on all diets except the regrowth ryegrass. Losses were 0.30 and 0.40 g/g N intake on the primary growth ryegrass and the regrowth clover respectively; N contents were 37 and 39 g/kg DM respectively. 5. Comparison of the values obtained at pasture with that obtained when similar diets were offered to housed cattle (Beever et al. 1985) indicated that combined relations relating duodenal OM and non-ammonia-N (NAN) flows to OM and N intakes respectively could be established for each diet. For two diets (primary-growth grass and regrowth clover) the relations were curvilinear; for the remaining diets, the derived relations were linear.(ABSTRACT TRUNCATED AT 400 WORDS)
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http://dx.doi.org/10.1079/bjn19880083 | DOI Listing |
J Mammary Gland Biol Neoplasia
January 2025
Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Fluorescent biosensors offer a powerful tool for tracking and quantifying protein activity in living systems with high temporospatial resolution. However, the expression of genetically encoded fluorescent proteins can interfere with endogenous signaling pathways, potentially leading to developmental and physiological abnormalities. The EKAREV-NLS mouse model, which carries a FRET-based biosensor for monitoring extracellular signal-regulated kinase (ERK) activity, has been widely utilized both in vivo and in vitro across various cell types and organs.
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January 2025
Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
We have recently shown that fluoxetine (FX) suppressed polyinosinic-polycytidylic acid-induced inflammatory response and endothelin release in human epidermal keratinocytes, via the indirect inhibition of the phosphoinositide 3-kinase (PI3K)-pathway. Because PI3K-signaling is a positive regulator of the proliferation, in the current, highly focused follow-up study, we assessed the effects of FX (14 µM) on the proliferation and differentiation of human epidermal keratinocytes. We found that FX exerted anti-proliferative actions in 2D cultures (HaCaT and primary human epidermal keratinocytes [NHEKs]; 48- and 72-h; CyQUANT-assay) as well as in 3D reconstructed epidermal equivalents (48-h; Ki-67 immunohistochemistry).
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Ultrasound Obstet Gynecol
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Discipline of Obstetrics, Gynaecology and Neonatology, Faculty of Medicine and Health, University of Sydney, Sydney, NSW, Australia.
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