AI Article Synopsis
- Photobiocatalysis is an eco-friendly method that utilizes solar energy to facilitate redox enzymatic reactions without the need for expensive cofactors.
- A new system was developed using natural flavins as a photosensitizer to drive human cytochrome P450 (CYP) reactions with visible light, showcasing its effectiveness both in vitro and in whole-cell systems.
- The study found that various factors like substrate type and the presence of catalase influence the photobiocatalytic activity of CYP2E1, indicating broad potential for applying this solar-powered method to other human CYPs.
Article Abstract
Photobiocatalysis is a green platform for driving redox enzymatic reactions using solar energy, not needing high-cost cofactors and redox partners. Here, a visible light-driven whole-cell platform for human cytochrome P450 (CYP) photobiocatalysis was developed using natural flavins as a photosensitizer. Photoexcited flavins mediate NADPH/reductase-free, light-driven biocatalysis by human CYP2E1 both in vitro and in the whole-cell systems. In vitro tests demonstrated that the photobiocatalytic activity of CYP2E1 is dependent on the substrate type, the presence of catalase, and the acid type used as a sacificial electron donor. A protective effect of catalase was found against the inactivation of CYP2E1 heme by H O and the direct transfer of photo-induced electrons to the heme iron not by peroxide shunt. Furthermore, the P450 photobiocatalysis in whole cells containing human CYPs 1A1, 1A2, 1B1, and 3A4 demonstrated the general applicability of the solar-powered, flavin-mediated P450 photobiocatalytic system.
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| http://dx.doi.org/10.1002/cssc.202100944 | DOI Listing |
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