AI Article Synopsis
- The study examined the effects of x-irradiation on two human head and neck squamous carcinoma cell lines, 183A and 1483, focusing on cytotoxicity and DNA damage.
- The 1483 cell line showed 15 times more resistance to x-ray damage compared to the sensitive 183A line, which experienced twice the number of DNA double-strand breaks.
- Additionally, there was no increase in poly(adenosine diphosphoribose)-synthesis in the 183A cells, while a significant increase was observed in the resistant 1483 cells, suggesting this synthesis may influence sensitivity to radiation.
Article Abstract
Cytotoxicity, extent of DNA double-strand breaks, and stimulation of poly(adenosine diphosphoribose)-synthesis were measured in two established human head and neck squamous carcinoma cell lines (183A and 1483) following x-irradiation. The 1483 cell line was 15-fold more resistant to x-ray-mediated cytotoxicity than was the 183A cell line. X-ray-mediated DNA strand cleavage also differed in these two cell lines with the absolute frequency of DNA double-strand breaks in the sensitive cells 183A cells being twice that in the resistant 1483 cell line. No detectable stimulation of poly(adenosine diphosphoribose)-synthesis was measured in the sensitive 183A cells whereas a marked increase in incorporation of [3H]-nicotinamide adenine dinucleotide was readily detected following x-irradiation of the resistant 1483 cells. These findings suggest a possible role of altered poly(adenosine diphosphoribose)-synthesis in the sensitivity of human head and neck squamous carcinoma cells to ionizing radiation.
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| http://dx.doi.org/10.1016/0006-291x(88)90245-8 | DOI Listing |
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A possible role for altered poly(adenosine diphosphoribose)-synthesis in the sensitivity of human head and neck squamous carcinoma cells to ionizing radiation.
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August 1988
Department of Medical Oncology, University of Texas M. D. Anderson Hospital and Tumor Institute, Houston.
Article Synopsis
- The study examined the effects of x-irradiation on two human head and neck squamous carcinoma cell lines, 183A and 1483, focusing on cytotoxicity and DNA damage.
- The 1483 cell line showed 15 times more resistance to x-ray damage compared to the sensitive 183A line, which experienced twice the number of DNA double-strand breaks.
- Additionally, there was no increase in poly(adenosine diphosphoribose)-synthesis in the 183A cells, while a significant increase was observed in the resistant 1483 cells, suggesting this synthesis may influence sensitivity to radiation.
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