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Horizontally Acquired nrDNAs Persist in Low Amounts in Host Genomes and Evolve Independently of Native nrDNA. | LitMetric

AI Article Synopsis

  • Nuclear ribosomal DNA (nrDNA) in plant species, particularly in the barley genus, exhibits significant variations and dynamics due to horizontal transfer, where species acquire nrDNA from other genera.
  • Research using quantitative PCR revealed an extensive copy number variation (CNV) in native nrDNA ribotypes, with a marked difference among various taxa, yet no correlation to phylogeny or genome size.
  • Foreign nrDNA types constituted a minor percentage of the total nrDNA copies, indicating that native and non-native nrDNAs may evolve independently and do not affect each other's copy number.

Article Abstract

Nuclear ribosomal DNA (nrDNA) has displayed extraordinary dynamics during the evolution of plant species. However, the patterns and evolutionary significance of nrDNA array expansion or contraction are still relatively unknown. Moreover, only little is known of the fate of minority nrDNA copies acquired between species via horizontal transfer. The barley genus (Poaceae) represents a good model for such a study, as species of section acquired nrDNA via horizontal transfer from at least five different panicoid genera, causing long-term co-existence of native (-like) and non-native (panicoid) nrDNAs. Using quantitative PCR, we investigated copy number variation (CNV) of nrDNA in the diploid representatives of the genus . We estimated the copy number of the foreign, as well as of the native ITS types (ribotypes), and followed the pattern of their CNV in relation to the genus' phylogeny, species' genomes size and the number of nrDNA loci. For the native ribotype, we encountered an almost 19-fold variation in the mean copy number among the taxa analysed, ranging from 1689 copies (per 2C content) in subsp. to 31342 copies in subsp. . The copy numbers did not correlate with any of the genus' phylogeny, the species' genome size or the number of nrDNA loci. The CNV was high within the recognised groups (up to 13.2 × in the American I-genome species) as well as between accessions of the same species (up to 4×). Foreign ribotypes represent only a small fraction of the total number of nrDNA copies. Their copy numbers ranged from single units to tens and rarely hundreds of copies. They amounted, on average, to between 0.1% ( ribotype) and 1.9% ( ribotype) of total nrDNA. None of the foreign ribotypes showed significant differences with respect to phylogenetic groups recognised within the sect. . Overall, no correlation was found between copy numbers of native and foreign nrDNAs suggesting the sequestration and independent evolution of native and non-native nrDNA arrays. Therefore, foreign nrDNA in likely poses a dead-end by-product of horizontal gene transfer events.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165317PMC
http://dx.doi.org/10.3389/fpls.2021.672879DOI Listing

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