Detection of biotinylated nucleic acid hybrids by antibody-coated gold colloid.

Anal Biochem

Molecular Diagnostics Inc., Miles Research Center, West Haven, Connecticut 06516.

Published: May 1988

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Article Abstract

A simple and sensitive nonradioactive method for detecting biotinylated nucleic acid hybrids on nitrocellulose paper is described. The method employs anti-biotin antibody-coated gold colloid and silver enhancement. Comparative results indicate sensitivity similar to that of a commonly used enzymatic colorimetric detection method.

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http://dx.doi.org/10.1016/0003-2697(88)90145-5DOI Listing

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