AI Article Synopsis
- * It utilizes immunoassays (IgG and IgM) on dried blood spots and compares them with a reference test, showing high accuracy rates of 98.2% for IgG and 95.8% for IgM.
- * The findings suggest that dried blood samples are a practical and efficient alternative for screening in resource-limited settings, improving the management of toxoplasmosis in high-prevalence areas like Brazil.
Article Abstract
Objective: Most prenatal screening programs for toxoplasmosis use immunoassays in serum samples of pregnant women. Few studies assess the accuracy of screening tests in dried blood spots, which are of easy collection, storage, and transportation. The goals of the present study are to determine the performance and evaluate the agreement between an immunoassay of dried blood spots and a reference test in the serum of pregnant women from a population-based prenatal screening program for toxoplasmosis in Brazil.
Methods: A cross-sectional study was performed to compare the immunoassays Imunoscreen Toxoplasmose IgM and Imunoscreen Toxoplasmose IgG (Mbiolog Diagnósticos, Ltda., Contagem, Minas Gerais, Brazil)in dried blood spots with the enzyme-linked fluorescent assay (ELFA, BioMérieux S.A., Lyon, France) reference standard in the serum of pregnant women from Minas Gerais Congenital Toxoplasmosis Control Program.
Results: The dried blood spot test was able to discriminate positive and negative results of pregnant women when compared with the reference test, with an accuracy of 98.2% for immunoglobulin G (IgG), and of 95.8% for immunoglobulin M (IgM).
Conclusion: Dried blood samples are easy to collect, store, and transport, and they have a good performance, making this a promising method for prenatal toxoplasmosis screening programs in countries with continental dimensions, limited resources, and a high prevalence of toxoplasmosis, as is the case of Brazil.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10302860 | PMC |
| http://dx.doi.org/10.1055/s-0041-1730285 | DOI Listing |
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