AI Article Synopsis

  • Several human-pathogenic arenaviruses, like Lassa virus, cause serious illnesses and require handling in specialized, high-biosafety conditions, making safe inactivation crucial for further research.* -
  • A new protocol was developed using the less dangerous Morogoro arenavirus as a stand-in to test various inactivation methods while removing toxic chemicals before testing their effects in cell culture.* -
  • The study confirmed ten new inactivation techniques that effectively lowered virus levels to undetectable amounts, resulting in a solid and adaptable protocol for future arenavirus research and diagnostics.*

Article Abstract

Several of the human-pathogenic arenaviruses cause hemorrhagic fever and have to be handled under biosafety level 4 conditions, including Lassa virus. Rapid and safe inactivation of specimens containing these viruses is fundamental to enable downstream processing for diagnostics or research under lower biosafety conditions. We established a protocol to test the efficacy of inactivation methods using the low-pathogenic Morogoro arenavirus as surrogate for the related highly pathogenic viruses. As the validation of chemical inactivation methods in cell culture systems is difficult due to cell toxicity of commonly used chemicals, we employed filter devices to remove the chemical and concentrate the virus after inactivation and before inoculation into cell culture. Viral replication in the cells was monitored over 4 weeks by using indirect immunofluorescence and immunofocus assay. The performance of the protocol was verified using published inactivation methods including chemicals and heat. Ten additional methods to inactivate virus in infected cells or cell culture supernatant were validated and shown to reduce virus titers to undetectable levels. In summary, we provide a robust protocol for the validation of chemical and physical inactivation of arenaviruses in cell culture, which can be readily adapted to different inactivation methods and specimen matrices.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225210PMC
http://dx.doi.org/10.3390/v13060968DOI Listing

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