() is an aggressive opportunistic pathogen of prominent virulence and antibiotic resistance. These characteristics are due in part to the accessory gene regulator () quorum-sensing system, which allows for the rapid adaptation of to environmental changes and thus promotes virulence and the development of pathogenesis. AgrA is the system response regulator that binds to the P2 and P3 promoters and upregulates expression. In this study, we reveal that AgrA is modified by covalent binding of CoA (CoAlation) in response to oxidative or metabolic stress. The sites of CoAlation were mapped by liquid chromatography tandem mass spectrometry (LC-MS/MS) and revealed that oxidation-sensing Cys199 is modified by CoA. Surface plasmon resonance (SPR) analysis showed an inhibitory effect of CoAlation on the DNA-binding activity, as CoAlated AgrA had significantly lower affinity towards the P2 and P3 promoters than non-CoAlated AgrA. Overall, this study provides novel insights into the mode of transcriptional regulation in and further elucidates the link between the quorum-sensing and oxidation-sensing roles of the system.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228455 | PMC |
| http://dx.doi.org/10.3390/antiox10060841 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The mutational landscape of Staphylococcus aureus during colonisation.
Nat Commun
January 2025
Parasites & Microbes Programme, Wellcome Sanger Institute, Hinxton, UK.
Staphylococcus aureus is an important human pathogen and a commensal of the human nose and skin. Survival and persistence during colonisation are likely major drivers of S. aureus evolution.
View Article and Find Full Text PDFA Phase III, Double-Blind, Randomized, Multicenter, Clinical Trial to Evaluate the Efficacy and Safety of a Fixed-Dose Combination of Metformin Hydrochloride and Myo-Inositol Compared to Metformin in Patients With Polycystic Ovary Syndrome.
Cureus
December 2024
Department of Medical Affairs, Eris Lifesciences Ltd., Ahmedabad, IND.
Background Polycystic ovary syndrome (PCOS) poses a significant health concern among reproductive-aged women and is characterized by ovarian dysfunction, hyperandrogenism, and insulin resistance. This study aims to assess the efficacy and safety of metformin and myo-inositol combination therapy compared to metformin monotherapy in patients with PCOS. Materials and methods This was a phase III, double-blind, randomized controlled clinical trial.
View Article and Find Full Text PDFA Rare Case Report: Managing PCL Avulsion Fracture with a Fracture of the Proximal Tibial Shaft.
J Orthop Case Rep
January 2025
Department of Orthopaedics, SN Medical College, Agra, Uttar Pradesh, India.
Introduction: Posterior cruciate ligament (PCL) avulsion fractures of the tibia with ipsilateral tibial shaft represent a rare but challenging orthopedic injury, necessitating careful consideration of surgical interventions for optimal outcomes. This case report presents the successful management of tibial shaft fracture (proximal 1/3rd junction) along with ipsilateral PCL avulsion fracture of tibia using a novel approach using open reduction internal fixation (ORIF) of tibial shaft with Locking compression plate (LCP) with cannulated cancellous screw and spiked washer fixation of PCL avulsion. There are no case reports or research articles available for the management of PCL avulsion fracture of tibia associated with ipsilateral tibial shaft fracture.
View Article and Find Full Text PDFHerbicide and irrigation management options in conventionally-tilled wheat: deciphering water and energy budgeting, and grain and monetary output in north-Indian plains.
Sci Rep
January 2025
Institute of Agricultural Sciences, Banaras Hindu University, India, Varanasi.
In South Asia, declining water tables due to increased irrigation and labor shortages for manual weeding pose significant challenges for wheat production. Additionally, herbicide resistance, often resulting from poor management practices, further complicates weed problems. The objective of this study was to assess the impacts of traditional irrigation regimens (IRs) and herbicide application on wheat crops.
View Article and Find Full Text PDFPerceptions of healthcare workers on implementing the 2022 WHO verbal autopsy instrument in rural India through the existing public health system.
J Family Med Prim Care
December 2024
Indian Council of Medical Research, Ansari Nagar, New Delhi, India.
Background: Cause-of-death (CoD) information is crucial for health policy formulation, planning, and program implementation. Verbal Autopsy (VA) is an approach employed for the collection and analysis of CoD estimates at the population level where medical certification of cause of death is low and, secondly, for integrating it with the existing public health system by utilizing the grassroots level workforce.
Objective: The study aims to understand the field perspectives on implementing the 2022 WHO VA instrument in rural India through the existing public health system.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!