Effect of Dietary Supplementation of Fermented Pine Needle Extract on Productive Performance, Egg Quality, and Serum Lipid Parameters in Laying Hens.

This study aimed to investigate the supplemental effects of fermented pine () needle extract (FPNE) in laying hen diets on productive performance, egg quality, and serum lipid metabolites. A total of 108 40-week-old Hy-line brown laying hens were randomly assigned to one of the three dietary treatment groups: (1) basal diet + 0 mL FPNE/kg diet (CON), (2) basal diet + 2.5 mL FPNE/kg diet (T1), or (3) basal diet + 5 mL FPNE/kg diet (T2) for 6 weeks. Each group consisted of four replicates of nine hens each. Feed and water provided ad libitum. Results showed that dietary supplementation of FPNE increased egg production percentage (linear, < 0.01 and quadratic, < 0.05), egg mass (linear, < 0.05), and feed intake (linear, < 0.05) during the entire experimental period. In addition, dietary inclusion of FPNE significantly increased the eggshell color (linear, < 0.01), egg yolk color (quadratic, < 0.01), and eggshell breaking strength (linear, < 0.05 and quadratic, < 0.05) while the Haugh unit decreased (quadratic, < 0.05). However, serum lipid profile did not differ among the dietary treatments ( > 0.05). Notably, antioxidant activity of egg yolk was improved by significantly decreasing the malondialdehyde content in egg yolks after 6 weeks of storage (linear, < 0.001 and quadratic, < 0.05). In summary, dietary inclusion of FPNE could improve laying performance and the antioxidant capacity of eggs.