AI Article Synopsis
- The study evaluated a vaccine based on whole-sporozoites and a nanoparticle adjuvant (EtSz-IMS1313) in one-day-old chicks to enhance protection against coccidiosis.
- The vaccine showed a high anticoccidial index (ACI of 186) compared to lower values in control groups, indicating its effectiveness in inducing immunity.
- Comparison of immune responses revealed similar antibody recognition patterns in rabbits and chicks, with specific polypeptides consistently targeted, suggesting potential for further B cell antigen identification.
Article Abstract
This study investigated protection against following the vaccination of chicks with 5.3 × 10 whole-sporozoites emulsified in the nanoparticle adjuvant IMS 1313 N VG Montanide™ (EtSz-IMS1313). One-day-old specific pathogen-free (SPF) chicks were subcutaneously injected in the neck with EtSz-IMS1313 on the 1st and 10th days of age. Acquired immunity was assayed through a challenge with 3 × 10 homologous sporulated oocysts at 21 days of age. The anticoccidial index (ACI) calculated for every group showed the effectiveness of EtSz-IMS1313 as a vaccine with an ACI of 186; the mock-injected control showed an ACI of 18 and the unimmunized, challenged control showed an ACI of -28. In a comparison assay, antibodies from rabbits and SPF birds immunized with EtSz-IMS1313 recognized almost the same polypeptides in the blotting of sporozoites and merozoites. However, rabbit antisera showed the clearest recognition pattern. Polypeptides of 120, 105, 94, 70, 38, and 19 kDa from both life cycle stages were the most strongly recognized by both animal species. The zoite-specific IgG antibodies from the rabbits demonstrated the feasibility for successful B cell antigen identification.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151427 | PMC |
| http://dx.doi.org/10.3390/ani11051344 | DOI Listing |
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