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Analysis of Synchronous and Asynchronous In Vitro Infections with Homologous Murine Norovirus Strains Reveals Time-Dependent Viral Interference Effects. | LitMetric

Analysis of Synchronous and Asynchronous In Vitro Infections with Homologous Murine Norovirus Strains Reveals Time-Dependent Viral Interference Effects.

Viruses

FARAH Research Centre, Faculty of Veterinary Medicine, Veterinary Virology and Animal Viral Diseases, Department of Infectious and Parasitic Diseases, Liège University, 4000 Liège, Belgium.

Published: May 2021

AI Article Synopsis

  • - Viral recombination in noroviruses enhances their evolution and diversity, but synchronous infection of multiple viruses in a single cell is rare, making delayed secondary infections more likely.
  • - The study focuses on how timing affects the infection of two strains of murine norovirus, MNV-1 WU20 and CW1, by comparing synchronized (coinfection) and staggered (superinfection) infections.
  • - Findings reveal that when there is a delay of one to two hours between infections, the initially infecting WU20 strain often dominates the viral population, suggesting timing creates a barrier to subsequent superinfections.

Article Abstract

Viral recombination is a key mechanism in the evolution and diversity of noroviruses. In vivo, synchronous single-cell coinfection by multiple viruses, the ultimate prerequisite to viral recombination, is likely to be a rare event and delayed secondary infections are a more probable occurrence. Here, we determine the effect of a temporal separation of in vitro infections with the two homologous murine norovirus strains MNV-1 WU20 and CW1 on the composition of nascent viral populations. WU20 and CW1 were either synchronously inoculated onto murine macrophage cell monolayers (coinfection) or asynchronously applied (superinfection with varying titres of CW1 at half-hour to 24-h delays). Then, 24 h after initial co-or superinfection, quantification of genomic copy numbers and discriminative screening of plaque picked infectious progeny viruses demonstrated a time-dependent predominance of primary infecting WU20 in the majority of viral progenies. Our results indicate that a time interval from one to two hours onwards between two consecutive norovirus infections allows for the establishment of a barrier that reduces or prevents superinfection.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147416PMC
http://dx.doi.org/10.3390/v13050823DOI Listing

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