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New Detection Platform for Screening Bacteria in Liquid Samples. | LitMetric

AI Article Synopsis

  • * The method includes three key steps: capturing bacteria with a specialized cryogel, detecting different types of bacteria using antimicrobial peptides (AMPs), and identifying specific bacterial strains with labelled antibodies.
  • * Testing showed that this approach effectively categorizes and identifies both Gram-positive and Gram-negative bacteria, potentially revealing a wide range of previously unknown bacterial species.

Article Abstract

The development of sensitive methods for the determination of potential bacterial contamination is of upmost importance for environmental monitoring and food safety. In this study, we present a new method combining a fast pre-enrichment step using a microporous cryogel and a detection and identification step using antimicrobial peptides (AMPs) and labelled antibodies, respectively. The experimental method consists of: (i) the capture of large amounts of bacteria from liquid samples by using a highly porous and functionalized cryogel; (ii) the detection and categorisation of Gram-positive and Gram-negative bacteria by determining their affinities toward a small set of AMPs; and (iii) the identification of the bacterial strain by using labelled detection antibodies. As proof of concept, the assessment of the three steps of the analysis was performed by using and as models for Gram-negative and Gram-positive bacteria, respectively. The use of AMPs with broad specificity combined with labelled antibodies enabled the detection and potential categorization of a large spectrum of unknown or unexpected bacteria.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147366PMC
http://dx.doi.org/10.3390/bios11050142DOI Listing

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