AI Article Synopsis
- Porous calcium carbonate/carboxymethylcellulose (CaCO/CMC) microspheres were created using a biomimetic method to immobilize lysozyme through adsorption.
- The size and structure of these microspheres were analyzed using transmitted electron microscopy (TEM) and zeta potential measurements, while the effectiveness of lysozyme immobilization was confirmed with Fourier transform infrared (FTIR) spectroscopy.
- The study found that the optimal conditions for lysozyme adsorption included a pH of 9.2 and a temperature of 25°C, achieving a maximum capacity of 450 mg/g, with further analysis indicating that the lysozyme retained its structure throughout the process.
Article Abstract
Porous calcium carbonate/carboxymethylcellulose (CaCO/CMC) microspheres were prepared by the biomimetic mineralization method for lysozyme immobilization via adsorption. The size and morphology of CaCO/CMC microspheres were characterized by transmitted electron microscopy (TEM) and zeta potential measurement. The lysozyme immobilization was verified by Fourier transform infrared (FTIR) spectroscopy. The effects of pHs and temperatures on lysozyme adsorption were investigated as well. It was revealed that CaCO/CMC microspheres could immobilize lysozyme efficiently via electrostatic interactions and a maximum adsorption capacity of 450mg/g was achieved at pH 9.2 and 25°C. Moreover, it was found that the adsorption process fitted well with the Langmuir isothermal model. In addition, UV, fluorescence, and circular dichroism (CD) spectroscopic studies showed that lysozyme maintained its original secondary structure during the adsorption/desorption process. Our study therefore demonstrated that CaCO/CMC microsphere can be used as a cost-effective and efficient support for lysozyme immobilization.
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| http://dx.doi.org/10.1016/j.msec.2012.05.027 | DOI Listing |
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