AI Article Synopsis
- The SH-SY5Y neuroblastoma cell line is commonly used to model molecular processes related to Alzheimer's disease (AD) due to its ability to differentiate into neurons and being cost-effective.
- Researchers found that undifferentiated SH-SY5Y cells do not accurately mimic the neuronal state affected by AD.
- The study reveals that fully differentiating SH-SY5Y cells using retinoic acid and BDNF significantly alters the expression and localization of amyloid-β protein precursor (AβPP), which is crucial for understanding AD pathology, and that insufficient differentiation leads to AβPP mislocalization.
Article Abstract
Neuroblastoma cell line SH-SY5Y, due to its capacity to differentiate into neurons, easy handling, and low cost, is a common experimental model to study molecular events leading to Alzheimer's disease (AD). However, it is prevalently used in its undifferentiated state, which does not resemble neurons affected by the disease. Here, we show that the expression and localization of amyloid-β protein precursor (AβPP), one of the key molecules involved in AD pathogenesis, is dramatically altered in SH-SY5Y cells fully differentiated by combined treatment with retinoic acid and BDNF. We show that insufficient differentiation of SH-SY5Y cells results in AβPP mislocalization.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385523 | PMC |
| http://dx.doi.org/10.3233/JAD-201409 | DOI Listing |
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