Background: World Health Organization (WHO) reported that more than 80% of people in the world use herbal traditional medicines nowadays. Many endemic medicinal plants, especially species, are facing to extinction as a result of high harvesting, limited distribution, and habitat destruction.Tissue culture is a successful method for plant secondary metabolites production. is a medicinal plant belonging to family Lamiaceae.
Objective: Our study was focused on devising an optimum procedure for callus induction and phenolic compounds production in . First, we are focused on finding suitable explants and media for callus induction. Then, subsequent experiments were conducted to find an optimal concentration of plant growth regulators (PGRs) and reduced- glutathione for maximum biomass production, and phenolic compounds production in calli.
Material And Method: In this study, the usage of whole plant grown in Hoagland nutrient solution, were used as a source of explants. Also, different media including, ½ MS, MS, and B5 and different combination of PGRs (NAA and BAP) were used for optimization of calli induction.
Results: Based on the results of the first experiment, leaf-originated explants, and macro half strength MS (½ MS) medium were used for the next experiments. The highest FW (Fresh Weight) and DW (Dry Weight) of calli were observed in ½ MS medium, supplemented with 2 μM/L reduced-glutathione, 2 mg.L BAP, and 2 mg.L NAA. The maximum amount of total phenolic, flavonoid, tannin contents and free-radical scavenger were observed in calli which were grown in ½ MS medium supplemented with 2 μM/L reduced-glutathione, 2 mg.L BAP, and 2 mg.L NAA.
Conclusion: Our study finds the optimum condition for calli induction and phenolic compounds production in .
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148645 | PMC |
| http://dx.doi.org/10.30498/IJB.2020.2621 | DOI Listing |
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