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Single-tube detection of a foodborne bacterial pathogen using user-friendly portable device.
Biosens Bioelectron
March 2025
School of Public Health, Jilin University, Changchun, 130021, PR China. Electronic address:
Timely and reliable detection of foodborne bacterial pathogen is crucial for reducing disease burden in low- and middle-income countries. However, laboratory-based methods are often inaccessibility in resource-limited settings. Here, we developed a single-tube assay and a low-cost palm-sized device for on-site detection of the representative foodborne bacterial pathogen, Salmonella Enteritidis.
View Article and Find Full Text PDFInfluences of Bacillus pumilus SA388 as an environmentally friendly antibiotic alternative on growth performance, blood biochemistry, immunology, cecal microbiota, and meat quality in broiler chickens.
Poult Sci
November 2024
Department of Agricultural Microbiology, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt.
The widespread use of antibiotics causes the development of antibiotic-resistant bacterial strains, which have a severe impact on poultry productivity and human health. As a result, research is continuing to develop safe natural antibiotic alternatives. In the current study, Bacillus pumilus SA388 was isolated from the chicken feces and confirmed to be a probiotic.
View Article and Find Full Text PDFProtein-Induced DNA Dumbbell Amplification (PINDA) and its applications to food hazards detection.
Biosens Bioelectron
December 2024
Division of Analytical and Environmental Toxicology, Faculty of Medicine and Dentistry, University of Alberta, 10-102 Clinical Sciences Building, Edmonton, Alberta, T6G 2G3, Canada. Electronic address:
Quantification of trace amounts of proteins is technically challenging because proteins cannot be directly amplified like nucleic acids. To improve the analytical sensitivity and to complement conventional protein analysis methods, we developed a highly sensitive and homogeneous detection strategy called Protein-Induced DNA Dumbbell Amplification (PINDA). PINDA combines protein recognition with exponential nucleic acid amplification by using protein binding probes made of DNA strands conjugated to protein affinity ligands.
View Article and Find Full Text PDFLevothyroxine synthesis impurities are neither mutagenic nor genotoxic: Insilico, Ames test and micronucleus test studies.
Mutat Res Genet Toxicol Environ Mutagen
August 2024
Shribios Innovations Pvt. Ltd. Pune, India.
In vitro and in silico tests were used to assess the possible genotoxicity and mutagenicity of five impurities that may be present in levothyroxine, a drug used for thyroid hormone replacement therapy. Neither ToxTree nor VEGA (Virtual Models for evaluating the properties of chemicals within a global architecture) identified cause for concern for any of the impurities. Ames test results (doses up to 1 mg per plate), with or without metabolic activation, were negative.
View Article and Find Full Text PDFFine-needle aspiration biopsy proven -induced mycotic aneurysm.
BMJ Case Rep
April 2024
Internal Medicine, Infectious Diseases, University of Iowa Hospitals and Clinics, Iowa City, IA, USA.
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