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Early pregnancy diagnosis in cows using corpus luteum blood flow analysis based on colour Doppler ultrasonography and mRNA analysis.
BMC Vet Res
December 2024
Department of Animal Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, 20400, Sri Lanka.
Background: Reproductive efficiency is paramount in the dairy industry, where early pregnancy detection of dairy cows will allow to detect the non-pregnant animals early, thus enabling to re-synchronize them and getting them pregnant leading to decrease in calving interval, which, in turn, is critical for maximizing productivity and economic gain. The objective of this study was to evaluate the colour Doppler ultrasonography (CDUS) and peripheral blood leukocytes (PBLs)-based pregnancy-associated biomarker mRNAs expression for the earliest detection of pregnancy status in the dairy cows at post insemination. Intensively managed animals were ovulation synchronized and subjected to timed artificial insemination (TAI).
View Article and Find Full Text PDFDevelopment of a 3D in vitro model to study corpus luteum of felids based on luteinized cells from antral follicles.
Cell Tissue Res
December 2024
Department of Reproduction Biology, Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Straße 17, 10315, Berlin, Germany.
The study aimed to establish a long-term 3D cell culture model using luteinized follicular cells to investigate the functionality and life cycle of the CL in felids. A mixture of cell types from antral follicles was luteinized in vitro and cultured for up to 23 days. The method, initially applied to the domestic cat, was later extended to Persian and Clouded leopards.
View Article and Find Full Text PDFReproductive outcomes of lactating dairy cows submitted to first timed-artificial insemination protocols with different strategies to induce final ovulation.
J Dairy Sci
December 2024
Department of Animal Science, University of São Paulo, Piracicaba, SP, Brazil, 13418-900. Electronic address:
The study evaluated strategies for induction of ovulation at the end of timed-artificial insemination (TAI) protocols initiated after a novel presynchronization strategy. A total of 909 lactating dairy cows from 6 dairy herds initiated a presynchronization protocol on d -15 with an intravaginal progesterone (P4) implant and 7 d later (d -8) were treated with 1.0 mg of estradiol cypionate (EC) and 0.
View Article and Find Full Text PDFConception rate according to the size and location of corpus luteum and coexistent follicle before embryo transfer in Hanwoo.
J Anim Sci Technol
November 2024
Department of Animal Science, Chonnam National University, Gwangju 61186, Korea.
The size and location of the corpus luteum and the presence of coexistent follicles are crucial factors in synchronizing recipients and determining the suitability for embryo transfer. However, there has been a recent decline in conception rates after embryo transfer, which is attributed to environmental pollution, uterine inflammation, ovarian cysts, and other factors. Therefore, we conducted experiments to establish a novel criterion for successful embryo transfer assessment.
View Article and Find Full Text PDFPresynchronization with a progesterone device and prostaglandin F2α enhances ovulatory response to first GnRH, estrus expression and tended to increase fertility in beef heifers submitted to a 5-day CO-Synch protocol.
Theriogenology
December 2024
Agrotecnio Center, Department of Animal Sciences, University of Lleida, Lleida, 25198, Spain. Electronic address:
The main objectives of the present study were to determine the effects of presynchronizing with a 1.0 g intravaginal progesterone device (IVPD) and prostaglandin F2α and to assess the effects of re-utilization of IVPD in a 2x2 factorial design, on the ovulatory response to first GnRH, ovarian status at different protocol stages, estrus expression and fertility in beef heifers submitted to a 5d-CO-Synch + Progesterone (P4) protocol. Beef heifers (n = 564) were assigned to 1 of 2 treatments at D-15: Pres5 (n = 283), where heifers received a (IVPD) for 5 days and administration of prostaglandin F2α (25 mg of dinoprost) at D-10; and Control (n = 281), where heifers received no treatment.
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