In order to find a new preservation solution for avian coccidial oocysts that can replace potassium dichromate (KCrO) solution, Eimeria tenella oocysts were preserved in 0.1 to 10% potassium sorbate (CHKO) solution in this study. The results showed that there was no significant difference between the sporulation rate of E. tenella oocysts preserved in 0.1 to 10% CHKO solution and in 2.5% KCrO solution (p > 0.05). The 0.5 to 10% CHKO solution could also effectively inhibit the growth of bacterial microorganisms. E. tenella oocysts preserved in 1% CHKO solution at 4 °C for 3, 6, 9, and 12 months, with the oocyst production of E. tenella oocysts being 1.3-, 1.2-, 1.6-, and 1.3-fold higher than that of oocysts stored in 2.5% KCrO solution (p < 0.05). In conclusion, CHKO could replace KCrO as the preservation solution of avian coccidial oocysts.
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http://dx.doi.org/10.1007/s00436-020-06792-3 | DOI Listing |
Acta Parasitol
January 2025
Graduate School of Veterinary Medical Sciences, Osaka Metropolitan University, 1-58 Rinku Orai Kita, Izumisano, Osaka, 598-8531, Japan.
Purpose: Flotation methods are widely used to detect oocysts/cysts of protozoans and eggs of helminths, except trematodes. However, details regarding the concentration and recovery rates of these parasites are poorly understood.
Methods: Using Eimeria tenella oocysts as a model parasite, the present study evaluated three check points: (1) the proportion of parasites that remain floating in flotation solution (sucrose or saturated saline) during centrifugation, (2) the proportion of oocysts that naturally float after addition of flotation solution after centrifugation, and (3) the rate of recovery on cover slips after completion of the flotation protocol.
Parasitol Res
December 2024
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
Coccidiosis is caused by apicomplexan parasites of the genus Eimeria, which infect epithelial cells of the intestinal tract causing diarrhea and negatively impacting production in the poultry industry. The self-limiting and highly immunogenic nature of infection by Eimeria spp. make live vaccination an effective means of coccidiosis control.
View Article and Find Full Text PDFAppl Environ Microbiol
December 2024
Department of Animal and Food Sciences, University of Delaware, Newark, Delaware, USA.
Unlabelled: Agricultural water is a potential source of microbial contamination whereby and can enter the food supply. To reduce this risk, effective sanitization of agricultural water may be critical to food safety. As such, it is important to investigate the effects of aqueous peracetic acid (PAA) and chlorine (Cl) on bacteria and protozoa at different treatment times and temperatures in agricultural water with respect to key water characteristics.
View Article and Find Full Text PDFParasit Vectors
November 2024
College of Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, People's Republic of China.
Background: Growing evidence indicates that N-methylguanosine (mG) modification plays critical roles in epigenetic regulation. However, no data regarding mG modification are currently available in Eimeria tenella, a highly virulent species causing coccidiosis in chickens.
Methods: In the present study, we explore the distribution of internal messenger RNA (mRNA) mG modification in sporulated and unsporulated oocysts of E.
Microorganisms
November 2024
Environmental Microbial and Food Safety Laboratory, Beltsville Agricultural Research Center, Northeast Area, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USA.
Recurring outbreaks of cyclosporiasis linked to fresh produce demonstrate the need to develop interventions to reduce in irrigation water. is resistant to commonly used irrigation water treatments, such as chemical sanitizers, making removal of oocysts by filtration the most suitable intervention. This study evaluated the reduction of and , as surrogates for , in water using filters packed with sand alone or mixtures of sand and zero-valent iron (ZVI).
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