Sporulation rate and viability of Eimeria tenella oocysts stored in potassium sorbate solution.

Parasitol Res

State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China.

Published: June 2021

In order to find a new preservation solution for avian coccidial oocysts that can replace potassium dichromate (KCrO) solution, Eimeria tenella oocysts were preserved in 0.1 to 10% potassium sorbate (CHKO) solution in this study. The results showed that there was no significant difference between the sporulation rate of E. tenella oocysts preserved in 0.1 to 10% CHKO solution and in 2.5% KCrO solution (p > 0.05). The 0.5 to 10% CHKO solution could also effectively inhibit the growth of bacterial microorganisms. E. tenella oocysts preserved in 1% CHKO solution at 4 °C for 3, 6, 9, and 12 months, with the oocyst production of E. tenella oocysts being 1.3-, 1.2-, 1.6-, and 1.3-fold higher than that of oocysts stored in 2.5% KCrO solution (p < 0.05). In conclusion, CHKO could replace KCrO as the preservation solution of avian coccidial oocysts.

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http://dx.doi.org/10.1007/s00436-020-06792-3DOI Listing

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