LncRNA RP11-390F4.3 inhibits invasion and migration of glioblastoma cells by downregulating ROCK1.

Background: This study aimed to investigate the role of lncRNA RP11-390F4.3 in glioblastoma.

Methods: The expression levels of RP11-390F4.3, miR-148a and ROCK1 in glioblastoma and nontumor tissues were measured by performing quantitative PCR (qPCR) and data were compared using paired t test. Linear regression analysis was performed to analyze the correlations between RP11-390F4.3 and miR-148a/ROCK1 in glioblastoma tissues. The effects of overexpression of RP11-390F4.3, miR-148a and ROCK1 on U-373 MG cell invasion and migration were analyzed by Transwell assay.

Results: RP11-390F4.3 and ROCK1 were both upregulated in glioblastoma, while miR-148a was downregulated in glioblastoma. In glioblastoma, RP11-390F4.3 was positively correlated with ROCK1 but negatively correlated with miR-148a. In glioblastoma cells, overexpression of RP11-390F4.3 led to upregulated ROCK1 and downregulated miR-148a. Cell invasion and migration analysis showed that overexpression of RP11-390F4.3 and ROCK1 resulted in increased, and overexpression of miR-148a resulted in deceased invasion and migration rates of glioblastoma cells.

Conclusion: Therefore, RP11-390F4.3 may upregulate ROCK1 by downregulating miR-148a to promote glioblastoma cell invasion and migration.