Identifying the factors and mechanisms that regulate metabolism under normal and diseased states requires methods to quantify metabolic fluxes of live tissues within their physiological milieu. A number of recent developments have expanded the reach and depth of isotope-based in vivo flux analysis, which have in turn challenged existing dogmas in metabolism research. First, minimally invasive techniques of intravenous isotope infusion and sampling have advanced in vivo metabolic tracer studies in animal models and human subjects. Second, recent breakthroughs in analytical instrumentation have expanded the scope of isotope labeling measurements and reduced sample volume requirements. Third, innovative modeling approaches and publicly available software tools have facilitated rigorous analysis of sophisticated experimental designs involving multiple tracers and expansive metabolomics datasets. These developments have enabled comprehensive in vivo quantification of metabolic fluxes in specific tissues and have set the stage for integrated multi-tissue flux assays.
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http://dx.doi.org/10.1016/j.copbio.2021.04.005 | DOI Listing |
Mol Biol Rep
December 2024
Faculty of Life and Natural Sciences, Department of Bioengineering, Abdullah Gül University, Sumer Campus, Kayseri, 38080, Turkey.
Background: Acute myeloid leukemia (AML) is a heterogeneous hematological malignancy caused by disorders in stem cell differentiation and excessive proliferation resulting in clonal expansion of dysfunctional cells called myeloid blasts. The combination of chemotherapeutic agents with natural product-based molecules is promising in the treatment of AML. In this study, we aim to investigate the anti-cancer effect of Rapamycin and Niacin combination on THP-1 and NB4 AML cell lines.
View Article and Find Full Text PDFACS Omega
December 2024
Guangdong Provincial Key Laboratory of Distributed Energy System, Dongguan University of Technology, Dongguan 523820, China.
To comprehensively explore syngas cocombustion technology, gasification experiments in a bench-scale circulating fluidized bed (CFB) and three-dimensional (3D) numerical simulations of a coal-fired boiler furnace have been conducted. In the amplification experiment of biomass gasification, sawdust has been gasified using air, oxygen-enriched air, and steam. The highest heating value of the syngas products reaches 12.
View Article and Find Full Text PDFUtilizing data from the Vitamin C, Thiamine, and Steroids in Sepsis (VICTAS) Trial, this hub model was developed to limit seventeen Renin-Angiotensin-Aldosterone System (RAAS) components as three entrance and four exits, to facilitate the calculation of a model as one egress unknown, the angiotensin type 1 (AT1) receptor. Following previous evidence relating renin levels to mortality, this study found controls were more like sepsis patients with levels < renin quartile 1 (Q1) for calculated AT1, while more like sepsis patients with renin levels > quartile 3 (Q4) for measured aldosterone levels. Additionally differential discrete correlate summation (DCS) analysis indicates AT1, aldosterone and renin as major hub nodes in this independent DCS model of metabolic data inputs.
View Article and Find Full Text PDFJ Hazard Mater
December 2024
School of Civil Engineering and Architecture, Taizhou University, Taizhou 318000, China. Electronic address:
The removal of selenite (Se(IV)) and cadmium (Cd(II)) from low-carbon wastewater presents significant challenges. However, the addition of external organic carbon sources is limited in application due to the high cost and potential for secondary pollution. This study introduced a "hibernation-like microbial survival strategy", enabling efficient removal of Se(IV) and Cd(II) in sulfur autotrophic reactor, with S acting as the electron donor.
View Article and Find Full Text PDFJ Environ Manage
December 2024
Nuclear and Engineering Nonproliferation Division, Los Alamos National Laboratory, Los Alamos, NM, 87545, USA.
Monitoring nuclear reactor operations is vital for nuclear safeguards as it ensures that reactors are in compliance with international legal agreements. Validating nuclear facilities and activities, including potential clandestine activities, is currently accomplished by using remotely sensed data from satellites and aircrafts and on-site sampling. However, these techniques are temporally-limited as sampling and interpretation of environmental releases frequently involve labor-intensive, on-site collections.
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