Avicenna J Phytomed
Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Published: January 2021
Objective: Chemoprevention of cancer by application of natural phytochemical compounds has been used to prevent, delay or suppress cancer progression. a traditional Iranian medicinal herb, has biological properties including anticancer, anti-aging, immuno-stimulatory and antioxidant effects. In this study, anti-proliferative effects of hydroalcoholic extract of on prostate (PC3) and breast (MCF7) cancer cell lines were investigated.
Materials And Methods: In the current study, we investigated treatment of PC3 cells with different concentrations of (0, 100, 200, 300, 400, and 500 µg/ml) for 24 and 48 hr; also, MCF7 cells were treated with various concentrations (0-600 µg/ml) of for 48 and 72 hr and cell viability was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. mRNA expression of BCL2 Associated X (), B-cell lymphoma 2 (), Cysteine-aspartic proteases () and Phosphatase and tensin homolog () were analyzed by quantitative real-time PCR. Annexin V/PI staining and lactate dehydrogenase (LDH) cytotoxicity assay were used to detect apoptosis.
Results: decreased PC3 and MCF7 cells viability in a dose- and time-dependent manner (p<0.01 to p<0.001). The gene expression of ratio, and increased in -treated cells compared to the control group. induced apoptosis (p<0.001) and LDH activity (p<0.01 to p<0.001).
Conclusion: Our findings suggest that extract is able to inhibit proliferation and induce apoptosis in PC3 and MCF7 cell lines. Therefore, extract exerts antitumor activity against cancer cells.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140209 | PMC |
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