AI Article Synopsis
- The study investigated the impact of trehalose on the freeze-drying process for preserving sperm, comparing it to traditional methods without trehalose.
- Results showed that sperm with trehalose maintained better viability, acrosome integrity, and DNA quality after freeze-drying, while both groups experienced total loss of motility.
- Although freeze-drying negatively affected sperm morphology, the presence of trehalose helped reduce some of the harmful effects associated with this preservation method.
Article Abstract
Freeze-drying is one of the sperm preservation methods leading to the long-term preservation of sperm genetic material. Our main goal of this study was to evaluate the effect of the trehalose freeze-drying method on sperm motility, viability, morphology, acrosome, and DNA integrity compared with a standard protocol without trehalose. Twenty-five normozoospermic samples were included in this prospective study. Direct swim-up was used for sperm preparation. An experiment was performed on freeze-dried samples containing trehalose (0.2 M), and the results were compared to that without trehalose. The sperm parameters, including count, motility, morphology, viability, acrosome reaction, DNA denaturation, and DNA fragmentation, were evaluated before and after freeze-drying in both groups. The spermatozoa were totally immotile after freeze-drying in both groups. Sperm viability, acrosome integrity, and nondenatured sperm DNA were significantly higher in the trehalose group in comparison with that of without trehalose group. Nonfragmented sperm DNA showed an increasing trend in the trehalose group compared to the group without trehalose. While freeze-drying significantly reduced normal morphology, the addition of trehalose did not affect this parameter. The results of this study showed that trehalose can attenuate the detrimental effects of freeze-drying on human sperm parameters.
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| http://dx.doi.org/10.1089/bio.2020.0167 | DOI Listing |
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