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Sulforaphane inhibits the expression of interleukin-6 and interleukin-8 induced in bronchial epithelial IB3-1 cells by exposure to the SARS-CoV-2 Spike protein. | LitMetric

Sulforaphane inhibits the expression of interleukin-6 and interleukin-8 induced in bronchial epithelial IB3-1 cells by exposure to the SARS-CoV-2 Spike protein.

Phytomedicine

Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy; Italian Consortium for Biotechnologies (C.I.B.); Research Center for Innovative Therapies of Cystic Fibrosis, University of Ferrara, Italy. Electronic address:

Published: July 2021

AI Article Synopsis

  • COVID-19 is linked to a severe inflammatory reaction called "cytokine storm," characterized by elevated levels of cytokines like IL-6 and IL-8, which can lead to Acute Respiratory Distress Syndrome (ARDS) in patients.
  • Sulforaphane (SFN), a compound found in certain vegetables, has anti-inflammatory properties and was tested to see if it could reduce levels of IL-6 and IL-8 in a laboratory setting.
  • The study found that SFN successfully inhibited the gene expression of IL-6 and IL-8 in bronchial cells exposed to SARS-CoV-2, suggesting that it might help manage the dangerous inflammatory responses in COVID-19 patients.

Article Abstract

Background: A key clinical feature of COVID-19 is a deep inflammatory state known as "cytokine storm" and characterized by high expression of several cytokines, chemokines and growth factors, including IL-6 and IL-8. A direct consequence of this inflammatory state in the lungs is the Acute Respiratory Distress Syndrome (ARDS), frequently observed in severe COVID-19 patients. The "cytokine storm" is associated with severe forms of COVID-19 and poor prognosis for COVID-19 patients. Sulforaphane (SFN), one of the main components of Brassica oleraceae L. (Brassicaceae or Cruciferae), is known to possess anti-inflammatory effects in tissues from several organs, among which joints, kidneys and lungs.

Purpose: The objective of the present study was to determine whether SFN is able to inhibit IL-6 and IL-8, two key molecules involved in the COVID-19 "cytokine storm".

Methods: The effects of SFN were studied in vitro on bronchial epithelial IB3-1 cells exposed to the SARS-CoV-2 Spike protein (S-protein). The anti-inflammatory activity of SFN on IL-6 and IL-8 expression has been evaluated by RT-qPCR and Bio-Plex analysis.

Results: In our study SFN inhibits, in cultured IB3-1 bronchial cells, the gene expression of IL-6 and IL-8 induced by the S-protein of SARS-CoV-2. This represents the proof-of-principle that SFN may modulate the release of some key proteins of the COVID-19 "cytokine storm".

Conclusion: The control of the cytokine storm is one of the major issues in the management of COVID-19 patients. Our study suggests that SFN can be employed in protocols useful to control hyperinflammatory state associated with SARS-CoV-2 infection.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8095027PMC
http://dx.doi.org/10.1016/j.phymed.2021.153583DOI Listing

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