AI Article Synopsis
- The protocol involves using automated confocal microscopy to perform live-cell high-throughput screening of yeast mutant strains that have fluorescent protein markers for specific subcellular compartments.
- This combined approach allows researchers to capture thousands of images systematically, enabling the identification of morphology defects in those compartments through quantitative analysis.
- The high-throughput protocol is flexible, allowing for the testing of various marker combinations and can be adjusted for different growth conditions or more complex genetic backgrounds.
Article Abstract
We describe a protocol for live-cell high-throughput (HTP) screening of yeast mutant strains carrying fluorescent protein markers for subcellular compartments of choice using automated confocal microscopy. This procedure, which combines HTP genetics and microscopy, results in the acquisition of thousands of images that can be analyzed in a systematic and quantitative way to identify morphology defects in the tagged subcellular compartments. This HTP protocol is readily adapted for screening any combination of markers and can be expanded to different growth conditions or higher order mutant genetic backgrounds.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107603 | PMC |
| http://dx.doi.org/10.1007/978-1-0716-1402-0_12 | DOI Listing |
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