AI Article Synopsis
- The study focuses on the mycobacterial acid-resistant protease (MarP), which plays a role in the survival of Mycobacterium tuberculosis within macrophages.
- Researchers expressed MarP in the yeast Pichia pastoris and examined its ability to modulate immune responses in human cells.
- Results showed that a partial version of MarP effectively induced the release of cytokines TNF-α and IL-10 from THP-1 cells, confirming its activity in vitro.
Article Abstract
Objective: Mycobacterial acid-resistant protease (MarP) is a membrane-associated serine protease involved in the survival of Mycobacterium tuberculosis in macrophages; here we produced MarP in the yeast Pichia pastoris and study its involvement in macrophage immune modulation.
Results: Pichia pastoris vectors, harboring a full-length or a partial sequence of MarP, were constructed. GS115 clones were selected, and homologous recombination at the AOX1 locus was assessed by PCR. Protein was purified by nickel affinity chromatography, and its effect on the cytokine profile was tested in human monocytes. Only the partial MarP protein (121-397 a.a.) lacking the transmembrane domain was successfully expressed as an N-glycosylated proteolytically active protease. In vitro stimulation of THP-1 cells with MarP promoted the release of TNF-α and IL-10.
Conclusion: Mycobacterial MarP was successfully expressed in P. pastoris, and it is capable of cytokine release in vitro.
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| http://dx.doi.org/10.1007/s10529-021-03149-3 | DOI Listing |
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