Low-molecular-weight thiols are widely present in human fluids, and are regarded as a kind of potential broad-spectrum evaluation indicators for some clinical diseases. In this work, gold nanoparticles capped with Tween 20 were used for purification and microextraction of the main free thiols (cysteine, homocysteine, glutathione and methionine) in saliva based on Au-S bond formation. Ultrasound further sped up the releasing of the target analytes, and the releasing time needed was only 10 min, and the required sample volume was only 40 µL. The desorption solution could be directly injected for electrophoretic analysis without derivatization, and field-amplified sample stacking of electrophoretic online enrichment technology further improved the detection sensitivity. The synergistic enrichment effect made the enrichment factors of four analytes reach 1119-2067 times. This developed method was applied for the analyses of saliva samples of healthy volunteers. Acceptable sensitivity (LODs: 0.15-1.5 ng mL) and recoveries (97.6-116%) were obtained in the saliva sample matrix. This proposed method provides an alternative for the sensitive detection of low-molecular-weight thiols in noninvasive body fluids, which has potential application prospect in the preliminary noninvasive diagnosis of diabetes, cardiovascular diseases, etc.
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http://dx.doi.org/10.1016/j.jchromb.2021.122756 | DOI Listing |
Environ Pollut
December 2024
Shandong Key Laboratory of Environmental Processes and Health, School of Environmental Science and Engineering, Shandong University, Qingdao, 266237, China; Department of Chemistry & Biochemistry, Florida International University, Miami, FL, 33199, United States. Electronic address:
Recent advancements in mercury (Hg) isotopic fractionation research have evolved from conceptual demonstrations to practical applications. However, few studies have focused on revealing fractionation fingerprinting for aqueous methylmercury (MeHg) photodegradation due to its sensitivity to natural organic matter (NOM). Here, the impact of NOM fractions with varying chemical properties on MeHg photodegradation kinetics and Hg isotope fractionation characteristics was investigated.
View Article and Find Full Text PDFEcotoxicol Environ Saf
December 2024
The College of Landscape Architecture and Arts, Northwest A&F University, Yangling 712100, China. Electronic address:
Cadmium (Cd) contaminants with high toxicity and mobility seriously threatens the ecological environment and human safety. Hydrangea macrophylla is a potential plant for Cd-contaminated soil remediation. Exogenous organic acids have been proven to effectively enhance the phytoremediation of soil contaminated with Cd.
View Article and Find Full Text PDFSci Rep
November 2024
Global CMC Development, Global R&D, Sanofi, 350 Water Street, MA 02141, Cambridge, USA.
Applications of functionalized hyaluronic acid (HA) hydrogels for numerous biomedical applications requires their detailed structural characterization. Since these materials are prepared by multistep chemical modifications in the solid phase and not amenable to characterization by standard analytical tools, we employed high-resolution solid-state NMR spectroscopy to gain detailed insights into the structures of the functionalized HA hydrogels. Divinyl sulfone crosslinked HA hydrogels were converted into maleimide-functionalized hydrogels, which were subjected to chemoselective thiol-maleimide reaction using L-cysteine as the protein mimetic thiol reagent.
View Article and Find Full Text PDFJ Agric Food Chem
November 2024
Analytical Chemistry Department. Faculty of Chemical Sciences, Complutense University of Madrid, Madrid 28040, Spain.
Two potent antioxidants, selenoneine and ergothioneine, have been determined simultaneously for the first time in the muscle of highly consumed fish and in fish-derived products. The extraction methods were optimized, and their subsequent analysis was performed by HPLC-ESI-MS/MS. Best extraction efficiency (106 ± 3 and 104 ± 5%) was attained by performing a simple aqueous extraction containing 100 mmol L ammonium acetate or ammonium formate as extractants.
View Article and Find Full Text PDFEcotoxicol Environ Saf
November 2024
Shandong Key Laboratory of Environmental Processes and Health, School of Environmental Science and Engineering, Shandong University, Qingdao, Shandong 266237, PR China; Sino-French Research Institute for Ecology and Environment (ISFREE), Shandong University, Qingdao, Shandong 266237, PR China.
The challenge of understanding the interaction between trace elements and microbial life is critical for assessing environmental and ecological impacts. Nevertheless, cysteine (Cys), a low molecular weight thiol substance prevalent in the ecosystem, is able to influence the fate of certain trace elements, which increases the complexity of the interaction between trace elements and microorganisms. Therefore, we chose Cys, selenite and the model methanogenic archaeon Methanosarcina acetivorans C2A as research targets, and comprehensively explored the intricate role of Cys in modulating the biological effects of selenite on M.
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