The virulence of eukaryotic parasites like depends on their capacity to escape from the host immune response and disseminate throughout the host organism. However, gene products essential for its pathogenesis remain uncharacterized. Here, we present the complete workflow of a CRISPR-Cas9 loss-of-function screen to identify fitness-conferring genes. This protocol can be used to uncover gene products that play a role in immune evasion, nutrient acquisition, dissemination, and tissue colonization. For complete details on the use and execution of this protocol, please refer to Sangaré et al. (2019).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113973 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2021.100520 | DOI Listing |
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