AI Article Synopsis
- The levels of 5-methyl-2'-deoxycytidine and its oxidized forms in mammalian cells indicate important insights about cellular conditions and future behavior.
- Advanced LC-MS techniques are capable of accurately measuring these specific DNA modifications at very low concentrations.
- This document outlines a general method for quantifying mdC, hmdC, fdC, and cadC in cells derived from vertebrates, employing ultra-HPLC triple quadrupole mass spectrometry.
Article Abstract
The amount of 5-methyl-2'-deoxycytidine (mdC) and its oxidized derivatives 5-hydroxymethyl-dC (hmdC), 5-formyl-dC (fdC), and 5-carboxy-dC (cadC) inside mammalian cells provides valuable information concerning cellular state and fate. LC-MS methods enable reliable quantification of these noncanonical DNA modifications in the low femtomolar range. Here, we describe a broadly applicable protocol to quantify mdC, hmdC, fdC, and cadC in vertebrate-derived cells using ultra-HPLC triple quadrupole MS (UHPLC-QQQ-MS).
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| http://dx.doi.org/10.1007/978-1-0716-1294-1_6 | DOI Listing |
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