There are three essential steps involved in bioethanol production from lignocellulosic biomass feedstocks. They are pretreatment, hydrolysis, and fermentation process. Among them, biomass pretreatment is an expensive and energy-intensive process used to remove the lignin and make the feedstock amenable for bioethanol production. The hydrodynamic cavitation can also be used for biomass pretreatment process. In order to improve the effectiveness of biomass pretreatment, a combination of any two methods of physical, chemical, and biological pretreatment can be used. A combination of the hydrodynamic cavitation pretreatment of biomass with the chemical or biochemical catalyst can be performed better than the individual pretreatment method. In this chapter, a protocol is describes the biomass pretreatment via a combined hydrodynamic cavitation with biocatalyst process.
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