Measurement of loss of resistance in glass syringes is a method widely used to locate the epidural space in epidural anaesthesia. Static and dynamic forces were measured under four experimental conditions in new glass syringes: unpolished, dry; polished, dry; unpolished, saline lubricated; and polished, saline lubricated. The unpolished saline lubricated syringes had a mean (SD) static force of 53.18 (15.0) g and dynamic force of 40.88 (15.2) g. These values were significantly greater than for polished dry syringes where the values were 5.27 (2.1) g and 4.38 (0.94) g, respectively. The results show that the least amount of resistance to plunger movement is obtained by dry polishing glass syringes.
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Biofabrication
January 2025
Research Group Anatomy, School for Medicine and Health Science, Carl von Ossietzky Universität Oldenburg, Carl von Ossietzky Str.9-11, Oldenburg, 26129, GERMANY.
Inkjet printing techniques are often used for bioprinting purposes because of their excellent printing characteristics, such as high cell viability and low apoptotic rate, contactless modus operandi, commercial availability, and low cost. However, they face some disadvantages, such as the use of bioinks of low viscosity, cell damage due to shear stress caused by drop ejection and jetting velocity, as well as a narrow range of available bioinks that still challenge the inkjet printing technology. New technological solutions are required to overcome these obstacles.
View Article and Find Full Text PDFNat Protoc
January 2025
Department of Molecular Metabolism, Harvard T.H. Chan School of Public Health, Boston, MA, USA.
Premetastatic cancer cells often spread from the primary lesion through the lymphatic vasculature and, clinically, the presence or absence of lymph node metastases impacts treatment decisions. However, little is known about cancer progression via the lymphatic system or of the effect that the lymphatic environment has on cancer progression. This is due, in part, to the technical challenge of studying lymphatic vessels and collecting lymph fluid.
View Article and Find Full Text PDFInt J Pharm
January 2025
Amgen Inc., Process Development, Manufacturing Technology Advancement (MTA) Group, Thousand Oaks, CA 91320, USA. Electronic address:
Animal Model Exp Med
January 2025
National Research Centre for the Working Environment, Copenhagen, Denmark.
Background: Inhalation exposure is the gold standard when assessing pulmonary toxicity. However, it typically requires substantial amounts of test material. Intratracheal instillation is an alternative administration technique, where the test substance is suspended in a liquid vehicle and deposited into the lung via the trachea.
View Article and Find Full Text PDFLab Chip
December 2024
Laboratory of Cell Biology and Histology, Faculty of Biomedical, Pharmaceutical and Veterinary sciences, University of Antwerp, Universiteitsplein 1, Antwerp, Belgium.
Modern cell and developmental biology increasingly relies on 3D cell culture systems such as organoids. However, routine interrogation with microscopy is often hindered by tedious, non-standardized sample mounting, limiting throughput. To address these bottlenecks, we have developed a pipeline for imaging intact organoids in flow, utilizing a transparent agarose fluidic chip that enables efficient and consistent recordings with theoretically unlimited throughput.
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