AI Article Synopsis

  • Microbial production of N-acetyl-glucosamine is becoming competitive, but glucose consumption limits yield due to competition with central carbon metabolism.
  • The study developed a strain (GLALD-7) that effectively utilized glycerol for cell growth while saving glucose for N-acetyl-glucosamine synthesis by optimizing the ratio of glycerol to glucose.
  • This strain achieved record production levels of 179.7 g/L N-acetyl-glucosamine in a bioreactor, indicating that managing carbon source utilization can enhance microbial chemical production.

Article Abstract

Currently, microbial production is becoming a competitive method for -acetyl-glucosamine production. As the biosynthesis of -acetyl-glucosamine originating from fructose-6-P directly competes with central carbon metabolism for precursor supply, the consumption of glucose for cell growth and cellular metabolism severely limits the yield of -acetyl-glucosamine. In this study, appropriate catabolic division of labor in the utilization of mixed carbon sources was achieved by deleting the gene and enhancing the utilization of glycerol by introducing the mutant. Glycerol thus mainly contributed to cell growth and cellular metabolism, and more glucose was saved for efficient -acetyl-glucosamine synthesis. By optimizing the ratio of glycerol to glucose, the balancing of cell growth/cellular metabolism and -acetyl-glucosamine synthesis was achieved. The resulting strain GLALD-7 produced 179.7 g/L -acetyl-glucosamine using mixed glycerol/glucose (1:8, m/m) carbon sources in a 5 L bioreactor, with a yield of 0.458 g/g total carbon sources (0.529 g/g glucose) and a productivity of 2.57 g/L/h. Coherent high titer/yield/productivity was obtained, with the highest values ever reported, suggesting that an appropriate catabolic division of labor using mixed glycerol/glucose carbon sources is a useful strategy for facilitating the microbial production of chemicals originating from glucose or metabolites upstream of glycolysis.

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Source
http://dx.doi.org/10.1021/acs.jafc.1c01513DOI Listing

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