We attempted to conduct an intraspecific analysis of 30 peony cultivars in Japan and to authenticate five herbal medicine samples derived from Paeoniae Radix by polymorphism analysis of the TEOSINTE BRANCHED1, CYCLOIDEA, and PCF (TCP) gene region. We focused on cultivar-dependent differences in leaf margin undulation and analyzed the sequence of the related TCP gene region. As a result, we found that the nucleotide sequences of 29 cultivars of Paeonia lactiflora except 'America' exhibit interspecific variations compared with the nucleotide sequences of Paeonia suffruticosa and Paeonia tenuifolia. Therefore, in the dendrogram constructed on the basis of the sequence similarity in the TCP gene region, the 29 cultivars of P. lactiflora were separated from P. suffruticosa, P. tenuifolia, and 'America', and clustered into three subgroups. There were 16 variations containing heterogenous DNA sequences within P. lactiflora species, and two characteristic variations in subgroup I. Some P. lactiflora cultivars showed the same nucleotide sequence in the TCP gene region, whereas the five herbal medicine samples showed different sequences, although all of them could be authenticated. The results suggest that Paeoniae Radix in the Japanese crude drug market can be authenticated by analysis of the TCP gene region.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s11418-021-01521-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comprehensive Analysis of the Gene Family and Its Expression Under UV-B Radiation in Bat.
Int J Mol Sci
December 2024
State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China.
TCP is a plant-specific transcription factor that plays an important role in plant growth and development. In this study, we used bioinformatics to identify the entire genome of the gene family in Bat, and we analyzed the expression characteristics of genes under UV-B radiation using qRT-PCR. The results were as follows: (1) 24 members of the gene family were identified in , evenly distributed on its 24 chromosomes.
View Article and Find Full Text PDFGenome-Wide Identification and Analysis of Transcription Factor Family Genes Involved in Cold Stress Tolerance in Winter Rapeseed ( L.).
Int J Mol Sci
December 2024
State Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
Article Synopsis
- TCP transcription factors are crucial for plant growth and stress responses, but their function in the cold stress response of L. is not well understood.
- This research identified a gene family in L., revealing 19 members with varying molecular weights and unstable proteins localized in the nucleus.
- The study found that these genes contain elements responsive to hormones, light, and stress, with significant differential expression during cold stress and other conditions, suggesting they are key regulators in stress response mechanisms.
Correlation of SALL1 with CEUS Parameters and Immune Escape in Thyroid Carcinoma.
Biomed J
January 2025
Department of Medical imaging, Henan Provincial People's Hospital, No. 7, Weiwu Road, Jinshui District, Zhengzhou, Henan, 450000, China.
Background: Contrast-enhanced ultrasonography (CEUS) is widely used to diagnose thyroid carcinoma (TC), though its accuracy in differentiating malignant nodules is limited. We identified TC-associated differentially expressed genes (DEGs) and examined the impact of these genes, particularly SALL1, on immune escape mechanisms within TC cells.
Methods And Materials: DEG analysis was conducted on GSE65144 dataset to identify genes associated with TC.
An injectable tricalcium silicate/α-tricalcium phosphate/hydroxypropyl methylcellulose biocomposite with improved physicochemical properties and osteoinductive activity for endodontic application.
Int J Biol Macromol
December 2024
School of Mechanical Engineering and Rail Transit, Changzhou University, Changzhou 213164, PR China.
Tricalcium silicate (TCS)-based bioactive cements have attracted great attention for various endodontic applications owing to their hydraulic property, sealing ability and biological properties. Nevertheless, poor handling property and anti-washout ability are the main challenges for traditional TCS-based cements and their osteoinductive capacity needs enhance for accelerated pulpal and periapical tissue repair/regeneration. Herein, we developed an injectable TCS/α-tricalcium phosphate (α-TCP)/hydroxypropyl methylcellulose (HPMC) biocomposite with improved physicochemical properties and osteoinductive ability via the incorporation of α-TCP/HPMC.
View Article and Find Full Text PDFMicrofluidic chip systems for characterizing glucose-responsive insulin-secreting cells equipped with FailSafe kill-switch.
Stem Cell Res Ther
December 2024
Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, ON, Canada.
Background: Pluripotent cell-derived islet replacement therapy offers promise for treating Type 1 diabetes (T1D), but concerns about uncontrolled cell proliferation and tumorigenicity present significant safety challenges. To address the safety concern, this study aims to establish a proof-of-concept for a glucose-responsive, insulin-secreting cell line integrated with a built-in FailSafe kill-switch.
Method: We generated β cell-induced progenitor-like cells (βiPLCs) from primary mouse pancreatic β cells through interrupted reprogramming.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!