Aminofutalosine synthase (MqnE) is a radical SAM enzyme that catalyzes the conversion of 3-((1-carboxyvinyl)oxy)benzoic acid to aminofutalosine during the futalosine-dependent menaquinone biosynthesis. In this Communication, we report the trapping of a radical intermediate in the MqnE-catalyzed reaction using sodium dithionite, molecular oxygen, or 5,5-dimethyl-1-pyrroline--oxide. These radical trapping strategies are potentially of general utility in the study of other radical SAM enzymes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.biochem.1c00181 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exploring the potential of soapstock over a glycerol in vitamin K2 production by Bacillus subtilis natto: a comparative analysis.
Microb Cell Fact
December 2024
Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, 1417864411, Iran.
Background: Vitamin K2 is an essential nutrient for blood coagulation and cardiovascular health and mainly produced by bacteria strain like B. subtilis. researchers have explored producing strain improvement, cultivation mode, environmental optimization, increased secretion, and using cheaper carbon and nitrogen sources in order to increase vitamin K2 productivity.
View Article and Find Full Text PDFUpdating the Relationship Between the Threshold Value of Average Nucleotide Identity and Digital DNA-DNA Hybridization for Reliable Taxonomy of .
Vet Sci
December 2024
Camel Research Center, King Faisal University, 400 Al-Ahsa, Hofuf 31982, Saudi Arabia.
Currently, bacterial classification at the species level relies on the 95-96% average nucleotide identity (ANI) value that is known to be equivalent to a 70% digital DNA-DNA hybridization (dDDH) value. However, during the routine identification of bacteria in the uteri of camels with a history of conception failure, we found that four out of the seven strains (2298A, 2569A, 2652, 2571B, 1103A, 2571A, and 335C) could not be assigned to any valid species. Furthermore, a 70% dDDH value did not correspond to a 95-96% ANI value in strain 2569A.
View Article and Find Full Text PDFProposal of Crystallibacter gen. nov., Crystallibacter permensis sp. nov. and Crystallibacter degradans sp. nov. for the salt-tolerant and aromatics degrading actinobacteria, and reclassification of Arthrobacter crystallopoietes as Crystallibacter crystallifaciens comb. nov.
Antonie Van Leeuwenhoek
December 2024
All-Russian Collection of Microorganisms (VKM), Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, 142290, Russia.
Four salt-tolerant and aromatics degrading strains used in this study were isolated from polluted technogenic soil on the territory of the Verkhnekamsk potash deposit (Russia). The strains were aerobic, Gram-stain-positive, non-motile, non-endospore-forming irregular rods, exhibiting a marked rod-coccus growth cycle. They contained lysine-based peptidoglycan, teichulosonic acid and poly(glycosyl phosphate) polymers in the cell walls.
View Article and Find Full Text PDFElucidating metabolic pathways through genomic analysis in highly heavy metal-resistant strains.
Heliyon
December 2024
Research Laboratory of Environmental Sciences and Sustainable Development, LR18ES32, University of Sfax, Tunisia.
The annotated and predicted genomes of five archaeal strains (AS1, AS2, AS8, AS11 and AS19), isolated from Sfax solar saltern sediments (Tunisia) and affiliated with , were performed by RAST webserver (Rapid Annotation using Subsystem Technology) and NCBI prokaryotic genome annotation pipeline (PGAP). The results showed the ability of strains to use a reduced semi-phosphorylative Entner-Doudoroff pathway for glucose degradation and an Embden-Meyerhof one for gluconeogenesis. They could use glucose, fructose, glycerol, and acetate as sole source of carbon and energy.
View Article and Find Full Text PDFDynamic quinone repertoire accompanied the diversification of energy metabolism in Pseudomonadota.
ISME J
January 2025
Univ. Grenoble Alpes, CNRS, UMR 5525, VetAgro Sup, Grenoble INP, TIMC, Grenoble 38000, France.
It is currently unclear how Pseudomonadota, a phylum that originated around the time of the Great Oxidation Event, became one of the most abundant and diverse bacterial phyla on Earth, with metabolically versatile members colonizing a wide range of environments with different O2 concentrations. Here, we address this question by studying isoprenoid quinones, which are central components of energy metabolism covering a wide range of redox potentials. We demonstrate that a dynamic repertoire of quinone biosynthetic pathways accompanied the diversification of Pseudomonadota.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!